Expression levels of ROS and Atg proteins in the vitreous in rhegmatogenous retinal detachment  

作　　者：Nan Huang Xue-Yan Gao Jin-Ping Li Xing Lu Hua-Mei Zhu Kai Dong 

机构地区：[1]Department of Ophthalmology,Xiaoxian People’s Hospital,Suzhou 234000,Anhui Province,China [2]Graduate School of Bengbu Medical College,Bengbu233000,Anhui Province,China [3]Department of Ophthalmology,Eye Center,the First AffiliatedHospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230001,Anhui Province,China [4]Graduate School of Anhui Medical University,Hefei 230001,Anhui Province,China [5]Hefei Aier Eye Hospital,Hefei 230001,Anhui Province,China

出　　处：《International Journal of Ophthalmology(English edition)》2023年第3期348-353,共6页国际眼科杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.82070977)。

摘　　要：AIM:To detect the concentrations of reactive oxygen species(ROS),transient receptor potential mucin-1(TRPML1),and autophagy-related(Atg)proteins(LC3-Ⅰ,LC3-Ⅱ,and Beclin1)in vitreous humor of patients with simple rhegmatogenous retinal detachment(RRD).METHODS:RRD patients enrolled as the RRD group,and patients with idiopathic macular hole(IMH)and idiopathic macular epiretinal membrane(IMEM)were enrolled as control group.The levels of ROS,TRPML1,LC3-Ⅰ,LC3-Ⅱ,and Beclin1 in vitreous humor of patients in the RRD and control groups were detected by enzyme-linked immunosorbent assay(ELISA).RESULTS:The RRD group included 28 eyes 28 patients and had a higher concentration of ROS in vitreous humor(631.86±18.05 vs 436.34±108.22 IU/m L,P<0.05).The ROS level in patients with a wide retinal detachment(RD)extent(RD range≥1/2)was higher than that with a narrow RD extent(RD range<1/2,P<0.05).ROS concentration was negatively correlated with RD time(r=-0.46,P=0.01).The expression levels of LC3-Ⅰand Beclin1 significantly decreased in RRD(P<0.05),but there were no correlations with the RD time,RD extent,or macular involvement.CONCLUSION:In eyes with RRD,the concentration of ROS in vitreous humor increases and the expression levels of Atg proteins decrease,reflecting possibly that autophagy is inhibited.

关 键 词：autophagy-related proteins reactive oxygen species vitreous body retinal detachment 

分 类 号：R774.12[医药卫生—眼科]