血小板对喉癌Hep-2细胞增殖、凋亡、侵袭和炎症细胞因子水平的影响  

Effect of platelet on the proliferation,apoptosis,invasion and inflammatory cytokines of laryngeal cancer cell Hep-2

作　　者：于小佳[1] 董航 赵丽君 薛浡 张晨光 YU Xiaojia;DONG Hang;ZHAO Lijun;XUE Bo;ZHANG Chenguang(Department of Otorhinolaryngology,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453003,Henan Province,China;School of Laboratory Medicine,Xinxiang Medical University,Xinxiang 453003,Henan Province,China;School of Public Health,Xinxiang Medical University,Xinxiang 453003,Henan Province,China)

机构地区：[1]新乡医学院第三附属医院耳鼻喉科,河南新乡453003 [2]新乡医学院医学检验学院,河南新乡453003 [3]新乡医学院公共卫生学院,河南新乡453003

出　　处：《新乡医学院学报》2023年第4期308-312,共5页Journal of Xinxiang Medical University

摘　　要：目的探讨血小板对喉癌细胞增殖、凋亡、侵袭和炎症细胞因子水平的影响。方法采集本课题组健康成年人全血20 mL,离心得到富血小板血浆(PRP);将PRP离心后收集上清液,即为乏血小板血浆(PPP),下层余下少许血浆为高血小板含量的PRP(简称为PRP2),血小板浓度为7.96×10^(11) L^(-1);将2 mL PRP2用PPP倍比稀释为低血小板含量的PRP(简称为PRP1),血小板浓度为4.25×10^(11) L^(-1)。将喉癌Hep-2细胞分为PPP组、PRP1组、PRP2组,分别培养于含体积分数10%PPP、PRP1、PRP2的培养基中。采用细胞计数试剂盒-8实验检测细胞增殖能力,Transwell小室实验检测细胞侵袭能力,流式细胞术检测细胞凋亡能力,实时荧光定量聚合酶链式反应法检测白细胞介素(IL)-1β、IL-6、IL-10、肿瘤坏死因子-α(TNF-α)mRNA表达。结果接种0 h时,3组细胞的增殖能力两两比较差异无统计学意义(P>0.05);接种24、48、72 h时,PRP1组、PRP2组细胞的增殖能力显著高于PPP组,PRP2组细胞的增殖能力显著高于PRP1组(P<0.05)。PPP组、PRP1组和PRP2组细胞侵袭数分别为374.67±16.26、548.00±39.51、917.00±16.70;3组细胞的侵袭能力比较差异有统计学意义(F=328.131,P<0.05);PRP1组、PRP2组细胞的侵袭能力显著高于PPP组,PRP2组细胞的侵袭能力显著高于PRP1组(P<0.05)。PPP组、PRP1组和PRP2组细胞凋亡率分别为(72.12±3.79)%、(10.57±1.44)%、(5.75±0.72)%;3组细胞的凋亡率比较差异有统计学意义(F=727.844,P<0.05);PRP1组、PRP2组细胞凋亡率显著低于PPP组,PRP2组细胞凋亡率显著低于PRP1组(P<0.05)。PRP1组、PRP2组细胞的IL-6、TNF-α、IL-1βmRNA相对表达量显著高于PPP组,IL-10 mRNA相对表达量显著低于PPP组(P<0.05);PRP2组细胞的IL-6、TNF-α、IL-1βmRNA相对表达量显著高于PRP1组,IL-10 mRNA相对表达量显著低于PRP1组(P<0.05)。结论血小板可以显著增强Hep-2细胞的增殖、侵袭能力,抑制其凋亡,且具有促炎作用。Objective To investigate the effect of platelet on the proliferation,apoptosis,invasion and inflammatory cytokines of laryngeal cancer cells.Methods A total of 20 mL of whole blood from healthy adults in the research group was collected and centrifuged to get platelet-rich plasma(PRP).The supernatant of PRP was collected after centrifugation,which was platelet-poor plasma(PPP);the rest of the lower plasma was PRP with high platelet content(PRP2),and the platelet concentration was 7.96×10^(11) L^(-1);2 mL of PRP2 was diluted with PPP multiple ratio to PRP with low platelet content(PRP1),and the platelet concentration was 4.25×10^(11) L^(-1).Laryngeal cancer Hep-2 cells were divided into PPP group,PRP1 group and PRP2 group,and cultured in the medium containing 10%PPP,PRP1 and PRP2,respectively.The cell prolife-ration ability was detected by cell counting kit-8 test;the cell invasion ability was detected by Transwell cell test;the cell apoptosis was detected by flow cytometry;the levels of interleukin(IL)-1β,IL-6,IL-10,tumor necrosis factor-α(TNF-α)mRNA were detected by real-time fluorescence quantitative polymerase chain reaction.Results At 0 h of inoculation,there was no significant difference in the proliferative ability of cells among the three groups(P>0.05);at 24,48 and 72 hours of inoculation,the proliferative capacity of cells in the PRP1 group and PRP2 group was significantly higher than that in the PPP group,and the proliferative capacity of cells in the PRP2 group was significantly higher than that in the PRP1 group(P<0.05).The number of cell invasion in the PPP group,PRP1 group and PRP2 group was 374.67±16.26,548.00±39.51 and 917.00±16.70,respectively;there was significant difference in the invasion ability of cells among the three groups(F=328.131,P<0.05);the invasion ability of cells in the PRP1 group and PRP2 group was significantly higher than that in the PPP group,and the invasion ability of cells in the PRP2 group was significantly higher than that in the PRP1 group(P<0.05).The apoptosis ra

关 键 词：喉癌 血小板 增殖 凋亡 侵袭 细胞因子 

分 类 号：R739.65[医药卫生—肿瘤]