急性髓系白血病患者外周血调节性T细胞、辅助性T细胞17及其相关细胞因子的表达及意义  

Expression and significance of regulatory T cells,helper T cell 17 cells and their related cytokines in peripheral blood of patients with acute myeloid leukemia

作　　者：张晓冬[1] 林晓媛[2] ZHANG Xiaodong;LIN Xiaoyuan(Department of Hematology,the First People′s Hospital of Nanyang City,Nanyang 473000,Henan Province,China;DepartmentⅡof Hematology,the First Affiliated Hospital of Nanyang Medical College,Nanyang 473000,Henan Province,China)

机构地区：[1]南阳市第一人民医院血液科,河南南阳473000 [2]南阳医学高等专科学校第一附属医院血液二科,河南南阳473000

出　　处：《新乡医学院学报》2023年第4期339-342,352,共5页Journal of Xinxiang Medical University

摘　　要：目的探讨急性髓系白血病(AML)患者外周血调节性T细胞(Treg)和辅助性T细胞17(Th17)及其相关细胞因子转化生长因子-β_(1)(TGF-β_(1))、白细胞介素(IL)-10和IL-17的表达及意义。方法选择2015年1月至2021年12月南阳市第一人民医院和南阳医学高等专科学校第一附属医院收治的85例AML患者为研究对象,根据《成人急性髓系白血病(非急性早幼粒细胞白血病)中国诊疗指南(2017年版)》预后危险度分级标准将患者分为非高危组(n=56)和高危组(n=29);另选择同期在本院体检的40例健康人作为对照组。应用流式细胞术检测3组受试者外周血Treg、Th17细胞水平,应用酶联免疫吸附法检测3组受试者血清中TGF-β_(1)、IL-10和IL-17水平。结果高危组和非高危组患者Treg细胞占外周血CD4^(+)T细胞百分比及Treg/Th17显著高于对照组,高危组患者Treg细胞占外周血CD4^(+)T细胞百分比及Treg/Th17显著高于非高危组(P<0.05);高危组和非高危组患者Th17细胞占外周血CD4^(+)T细胞百分比显著低于对照组,高危组患者Th17细胞占外周血CD4^(+)T细胞百分比显著低于非高危组(P<0.05)。高危组和非高危组患者血清中TGF-β_(1)、IL-10水平显著高于对照组,高危组患者血清中TGF-β_(1)、IL-10水平显著高于非高危组(P<0.05);高危组和非高危组患者血清中IL-17水平显著低于对照组,高危组患者血清中IL-17水平显著低于非高危组(P<0.05)。Pearson相关性分析结果显示,Treg细胞、Treg/Th17及血清TGF-β_(1)、IL-10水平与高危AML呈正相关(r=0.363、0.628、0.440、0.562,P<0.05),Th17细胞及血清IL-17水平与高危AML呈负相关(r=-0.473、-0.489,P<0.05)。结论AML患者Treg细胞呈高表达,Th17细胞呈低表达,Treg/Th17明显失衡,且该失衡现象在高危AML患者中更为明显。Objective To investigate the expression and significance of regulatory T cells(Treg),helper T cell 17(Th17)and their related cytokines transforming growth factor-β_(1)(TGF-β_(1)),interleukin(IL)-10 and IL-17 in peripheral blood of patients with acute myeloid leukemia(AML).Methods A total of 85 AML patients admitted to the First People′s Hospital of Nanyang City and the First Affiliated Hospital of Nanyang Medical College from January 2015 to December 2021 were selected as the research objects,and they were divided into the non-high-risk group(n=56)and high-risk group(n=29)according to the"Chinese Guidelines for Diagnosis and Treatment of Adult Acute Myeloid Leukemia(Non-Acute Promyelocytic Leukemia)(2017 Edition)";another 40 healthy people who underwent physical examination in our hospital during the same period were selected as the control group.The levels of Treg and Th17 cells in peripheral blood of subjects in the three groups were detected by flow cytometry,and the levels of TGF-β_(1),IL-10 and IL-17 in serum of the subjects in the three groups were detected by enzyme-linked immunosorbent assay.Results The percentage of Treg cells in CD4^(+)T cells in peripheral blood and Treg/Th17 of patients in the high-risk group and non-high-risk group were significantly higher than those in the control group,the percentage of Treg cells in CD4^(+)T cells in peripheral blood and Treg/Th17 of patients in the high-risk group were significantly higher than those in the non-high-risk group(P<0.05).The percentage of Th17 cells in CD4^(+)T cells in peripheral blood of patients in the high-risk group and non-high-risk group was significantly lower than that in the control group,and the percentage of Th17 cells in CD4^(+)T cells in peripheral blood of patients in the high-risk group was significantly lower than that in the non-high-risk group(P<0.05).The levels of TGF-β_(1) and IL-10 in serum of patients in the high-risk group and non-high-risk group were significantly higher than those in the control group,the levels of T

关 键 词：调节性T细胞 辅助性T细胞17 细胞因子 急性髓系白血病 

分 类 号：R733.71[医药卫生—肿瘤]