TLR4、TRAF6、IL-6和IL-1β在深静脉血栓大鼠模型中的表达和意义  

Expression and Significance of TLR4,TRAF6,IL-6 and IL-1βin Rat Model of Deep Vein Thrombosis

作　　者：王喜尧[1] 王育文[1] 齐桂云[1] 徐洪伟[1] 杜桂芹[1] WANG Xi-yao;WANG Yu-wen;QI Gui-yun;XU Hong-wei;DU Gui-qin(Department of Laboratory Medicine,The Second Affiliated Hospital of Harbin Medical University,Harbin,Heilongjiang,150086,China)

机构地区：[1]哈尔滨医科大学附属第二医院检验科,黑龙江哈尔滨150086

出　　处：《现代生物医学进展》2023年第4期641-645,共5页Progress in Modern Biomedicine

基　　金：黑龙江省卫生健康委项目(2019-235)。

摘　　要：目的:探究4个炎症因子Toll样受体4(TLR4)、TNF受体相关因子6(TRAF6)、白细胞介素-6(IL-6)和白细胞介素-1β(IL-1β)在深静脉血栓大鼠体内的表达及在深静脉血栓形成的作用。方法:将SD大鼠随机分成对照组(n=10)和模型组(n=90),采用血管钳夹结合固定制动的方式建立深静脉血栓模型,建模后分别于1 h、6 h、12 h、1 d、3 d、7 d、14 d、21 d、28 d处死,检测血栓重量/长度比值、血浆中4个炎症因子的浓度,并进行组织学观察。结果:(1)1 h、6 h、12 h的血栓重量/长度比值较低,组间无显著性差异(P>0.05);1 d、3 d、7 d的血栓重量/长度比值维持在较高水平,组间无显著性差异(P>0.05);14 d、21 d、28 d的血栓重量/长度比值均降低,组间无显著性差异(P>0.05)。(2)模型组中1-7 d的血栓重量/长度比值均明显高于正常组(P<0.05)。(3)模型组中1-7 d的TLR4、TRAF6、IL-6和IL-1β浓度水平均明显高于正常组(P<0.05)。(4)Pearson相关性分析显示TLR4、TRAF6、IL-6和IL-1β浓度与血栓程度具有正相关性。结论:深静脉血栓大鼠体内的TLR4、TRAF6、IL-6和IL-1β表达上调可能在深静脉血栓形成中发挥了重要作用。Objective:To investigate the effects of four inflammatory factors(Toll-like receptor 4,TLR4;TNF receptor-related factor 6,TRAF6;interleukin-6,IL-6;interleukin-1β,IL-1β)in rats with deep vein thrombosis expression and role in deep vein thrombosis.Methods:SD rats were randomly divided into control group(n=10)and model group(n=90),and the deep vein thrombosis model was established by vascular clamp combined with immobilization.After modeling,the rats were sacrificed at 1 h,6 h,12 h,1 d,3 d,7 d,14 d,21 d,and 28 d,respectively.The thrombus weight/length ratio and the concentration of 4 inflammatory factors in plasma were detected,and histological observation was performed.Results:(1)The thrombus weight/length ratio at 1 h,6 h,and 12 h was lower,and there was no significant difference between the groups(P>0.05);the thrombus weight/length ratio at 1 d,3 d,and 7 d remained at a high level,and there was no significant difference between the groups.The thrombus weight/length ratio decreased on 14 d,21 d,and 28 d,and there was no significant difference between groups(P>0.05).(2)The thrombus weight/length ratios in the model group from 1 to 7 days were significantly higher than those in the normal group(P<0.05).(3)The concentrations of TLR4,TRAF6,IL-6 and IL-1βin the model group from 1 to 7 days were significantly higher than those in the normal group(P<0.05).(4)Pearson correlation analysis showed that the concentrations of TLR4,TRAF6,IL-6 and IL-1βwere positively correlated with the degree of thrombosis.Conclusion:Up-regulation of TLR4,TRAF6,IL-6 and IL-1βin rats with deep vein thrombosis may play an important role in deep vein thrombosis.

关 键 词：深静脉血栓 TLR4 TRAF6 IL-6 IL-1Β 

分 类 号：R-33[医药卫生] R364.15