吸烟对种植体周围炎患者龈下菌群、龈沟液炎症因子和RANKL/OPG比值的影响  

Effects of Smoking on the Subgingival Microbiota,Gingival Crevicular Fluid Inflammatory Factors and RANKL/OPG Ratio in Patients with Periimplantitis

作　　者：丛锘锘[1] 颜兴[1] 李菁[1] 张宁[1] 苏莎[1] CONG Nuo-nuo;YAN Xing;LI Jing;ZHANG Ning;SU Sha(Department of Stomatology,Beijing Friendship Hospital Affiliated to Capital Medical University,Beijing,100050,China)

机构地区：[1]首都医科大学附属北京友谊医院口腔科,北京100050

出　　处：《现代生物医学进展》2023年第5期850-854,共5页Progress in Modern Biomedicine

基　　金：北京市自然科学基金项目(7202036)。

摘　　要：目的:探讨吸烟对种植体周围炎患者龈下菌群分布、龈沟液炎症因子白细胞介素-4(IL-4)、白细胞介素-5(IL-5)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、白细胞介素-17(IL-17)和核因子-κB受体活化因子配体/骨保护素(RANKL/OPG)比值的影响。方法:选择2019年3月至2022年3月首都医科大学附属北京友谊医院口腔科收治的种植体周围炎患者99例(共151颗种植体),根据是否吸烟分为吸烟组(45例,68颗种植体)和非吸烟组(54例,83颗种植体),比较两组患者种植体牙周改良菌斑指数(m PLI)、牙龈出血指数(GBI)、种植体周围探诊深度(PPD),采集两组龈下菌斑进行细胞培养并进行菌种鉴定,分析两组龈下菌群分布情况,比较两组龈沟液中IL-4、IL-5、IL-6、IL-8、IL-17、RANKL/OPG比值。结果:吸烟组m PLI、GBI、PPD高于不吸烟组(P<0.05)。吸烟组厌氧菌检出率高于非吸烟组(P<0.05),有益菌检出率低于非吸烟组(P<0.05),两组需氧菌检出率比较差异无统计学意义(P>0.05)。吸烟组龈沟液IL-4、IL-5、IL-6、IL-8、IL-17水平均高于非吸烟组(P<0.05)。吸烟组龈沟液RANKL水平、RANKL/OPG比值高于非吸烟组(P<0.05),OPG水平低于非吸烟组(P<0.05)。结论:吸烟可导致种植体周围炎患者龈下厌氧菌增加,加重炎症反应,增加牙槽骨吸收风险。Objective:To investigate the effects of smoking on the distribution of subgingival microbiota,gingival crevicular fluid interleukin-4(IL-4),interleukin-5(IL-5),interleukin-6(IL-6),interleukin-8(IL-8),interleukin-17(IL-17)and nuclear factor-kB receptor activator ligand/osteoprotegerin(RANKL/OPG)ratio in patients with perimplantitis.Methods:99 patients with perimplantitis(151 implants)who were admitted to the Stomatology Department of Beijing Friendship Hospital Affiliated to Capital Medical University from March 2019 to March 2022 were selected.According to whether they smoking,they were divided into smoking group(45 cases,68 implants)and non-smoking group(54 cases,83 implants).The periodontal modified plaque index(mPLI),gingival bleeding index(GBI)and peri-implant pocket depth(PPD)were compared between the two groups.Subgingival plaque was collected from the two groups for cell culture and bacterial species identification,and the distribution of subgingival microbiota was analyzed.The gingival crevicular fluid IL-4,IL-5,IL-6,IL-8,IL-17 and RANKL/OPG ratios of the two groups were compared.Results:The mPLI,GBI and PPD of the smoking group were higher than those of the non-smoking group(P<0.05).The detection rate of anaerobic bacteria of the smoking group was higher than that of the non-smoking group(P<0.05),The detection rate of beneficial bacteria of the smoking group was lower than that of non-smoking group(P<0.05),and there were no significant differences in the detection rate of aerobic bacteria between the two groups(P>0.05).The levels of gingival crevicular fluid IL-4,IL-5,IL-6,IL-8 and IL-17 of the smoking group were higher than those of the non-smoking group(P<0.05).The level of gingival crevicular fluid RANKL and RANKL/OPG ratio of the smoking group were higher than those of the non-smoking group(P<0.05),and the level of OPG was lower than that of the non-smoking group(P<0.05).Conclusion:Smoking can lead to the increase of subgingival anaerobic bacteria in patients with perimplantitis,aggravate inflammator

关 键 词：种植体周围炎 吸烟 龈下菌群 炎性因子 RANKL/OPG比值 

分 类 号：R783[医药卫生—口腔医学]