PCA3调控miR-185对霍奇金淋巴瘤细胞L428增殖和凋亡的影响  

Effect of PCA3 on Proliferation and Apoptosis of Hodgkin’s Lymphoma Cells L428 by Regulating miR-185

作　　者：刘慧[1] 王佳[1] 张虹丽[2] LIU Hui;WANG Jia;ZHANG Hongli(Department of Hematology,Hanzhong 3201th Hospital Affiliated to Xi’an Jiaotong University,Hanzhong,Shaanxi,723000,China;Department of Hematology,the Third Affiliated Hospital of Southern Medical University,Guangzhou,510665,China)

机构地区：[1]西安交通大学附属汉中三二○一医院血液内科,陕西省汉中市723000 [2]南方医科大学附属第三医院血液内科,广州市510665

出　　处：《医学分子生物学杂志》2023年第2期141-147,共7页Journal of Medical Molecular Biology

摘　　要：目的探讨PCA3对霍奇金淋巴瘤细胞L428增殖和凋亡的影响及可能机制。方法收集39例霍奇金淋巴瘤患者淋巴瘤组织和正常瘤旁组织,RT-qPCR检测组织中PCA3和miR-185表达水平。体外培养淋巴瘤L428细胞,分为si-NC组、si-PCA3组、si-PCA3+NC组、si-PCA3+miR-185 inhibitor组,CCK-8法检测细胞增殖,流式细胞仪检测细胞周期和细胞凋亡,RT-qPCR法检测PCA3和miR-185表达水平。双荧光素酶报告基因实验验证PCA3和miR-185的调控关系。结果淋巴瘤组织中PCA3的表达升高,miR-185的表达降低(P<0.05);转染si-PCA3可抑制细胞增殖,阻滞细胞周期,促进细胞凋亡。PCA3靶向负调控miR-185表达。共转染si-PCA3、miR-185 inhibitor可降低转染si-PCA3对细胞增殖、细胞周期、凋亡的作用。结论干扰PCA3表达可靶向负调控miR-185抑制淋巴瘤细胞L428增殖,并促进L428细胞凋亡。Objective To investigate the effect of PCA3 on the proliferation and apoptosis of Hodgkin’s lymphoma cell line L428 and its possible mechanism.Methods Lymphoma tissues and normal adjacent tissues from 39 patients with Hodgkin’s lymphoma were collected.The expression levels of PCA3 and miR-185 in the tissues were dectected by RT-qPCR.L428 cells were divided into si-NC group,si-PCA3 group,si-PCA3+NC group,si-PCA3+miR-185 inhibitor group.CCK-8 was used to detect cell proliferation.Flow cytometry was used to detect cell cycle and apoptosis.Western blotting was used to detect the protein expression levels of pro-caspase 3 and cleavedcaspase 3.RT-qPCR was used to detect the expression levels of PCA3 and miR-185.The dual luciferase gene reporter assay was used to verify the regulatory relationship between PCA3 and miR-185.Results The expression level of PCA3 was increased and the expression level of miR-185 was decreased in the lymphoma tissues(P<0.05).Transfection of si-PCA3 could inhibit cell proliferation,arrest cell cycle,and promote cell apoptosis in L428 cells.PCA3 targets to the miR-185 and negatively regulate its expression.Co-transfection of si-PCA3 and miR-185 inhibitor could reduce the effect of transfection of si-PCA3 on cell proliferation,cell cycle and apoptosis.Conclusion Interfering the expression of PCA3 could target and negatively regulate miR-185 to inhibit the proliferation of lymphoma cells L428 and promote the apoptosis of L428 cells.

关 键 词：霍奇金淋巴瘤 PCA3 miR-185 细胞增殖 凋亡 

分 类 号：R733.1[医药卫生—肿瘤]