A colorimetric assay for cholesterol based on the encapsulation of multienzyme in leaf-shape crossed ZIF-L  

作　　者：Jiaqi He Tingling Zhuo Yintong Teng Guoqin Chen Peng Zhao Caiwen Ou 

机构地区：[1]Department of Cardiology,Guangzhou Panyu Central Hospital,Guangzhou University of Chinese Medicine,Guangzhou 510006,China [2]Department of Cardiology and Laboratory of Heart Center,Zhujiang Hospital,Southern Medical University,Guangzhou 510280,China [3]NMPA Key Laboratory for Research and Evaluation of Drug Metabolism,Guangdong Provincial Key Laboratory of New Drug Screening,School of Pharmaceutical Sciences,Southern Medical University,Guangzhou 510515,China [4]Affiliated Dongguan Hospital,Southern Medical University,Dongguan 523059,China [5]Guangdong Provincial Key Laboratory of Shock and Microcirculation,Southern Medical University,Guangzhou 510515,China

出　　处：《Chinese Chemical Letters》2023年第2期382-387,共6页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. 81971765, 31771099, 31671025 and 81871504);the Natural Science Foundation of Guangdong Province (No. 2020A1515011066)。

摘　　要：The serum cholesterol level is an important indicator of healthy and there is a great necessity for frequent cholesterol monitoring to some cardiovascular-related diseases, which puts forward higher requirements for point-of-care testing(POCT) of cholesterol. In this work, a cascade catalytic system of cholesterol is developed by encapsulation of cholesterol oxidase(Ch Ox) and Pd Cu Au nanoparticles into zeolitic imidazolate framework-L(Ch Ox/PCA@ZIF-L). Results indicate that ZIF-L carrier can significantly increase the catalytic activity of single or multiple enzymes, due to its high loading capacity and efficient molecular transport. Under the optimal conditions, the absorbance of reaction system performs linear relationships with the concentration of cholesterol in two intervals from 0.0005 mmol/L to 1.0000 mmol/L, with a limit of detection of 0.2176 μmol/L. The proposed colorimetric strategy based on Ch Ox/PCA@ZIF-L performs a good agreement with the results provided by chemiluminescence method for the serum cholesterol detection. Interestingly, a simple paper-based sensing system is constructed through a pre-reaction-transfer operation, which gets rid of the complex pre-processing requirements of traditional operations on filter paper. The presented strategy allows for the sensitive, convenient, costless assay of serum cholesterol,and paves a new way to design the POCT device for daily monitoring of healthy.

关 键 词：CHOLESTEROL Zeolitic imidazolate framework-L Artificial enzyme COLORIMETRIC Point-of-care testing 

分 类 号：O657.3[理学—分析化学] R313[理学—化学]