The development of an iridium(Ⅲ) complex functionalized G-quadruplex probe for the stability of G-quadruplex and lifetime image in cytoplasm  

作　　者：Shaokang Jia Wenjin Wang Shanshan Qin Shengjie Xie Lisi Zhan Qi Wei Ziang Lu Xiaolu Zhou Cong Chen Kun Chen Shen Yan Caiping Tan Zongwan Mao Xiang Zhou 

机构地区：[1]College of Chemistry and Molecular Sciences,Key Laboratory of Biomedical Polymers-Ministry of Education,Wuhan University,Wuhan 430072,China [2]MOE Key Laboratory of Bioinorganic and Synthetic Chemistry,School of Chemistry,Sun Yat-sen University,Guangzhou 510275,China

出　　处：《Chinese Chemical Letters》2023年第2期397-402,共6页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. 92153303 and 21721005)。

摘　　要：G-quadruplex(G4) is widely known as a non-classical secondary structure of nucleic acid. With the indepth study of G4, it is an urgent need for a phosphorescent probe with a high G4 binding ability to evaluate the level of G4 in the cytoplasm. Thus, this study designed and synthesized Ir-PDP where an Ir(Ⅲ)complex was used as a phosphorescent emitter. Meanwhile, two installed PDPs(pyridostatin derivatives)were used to improve the combination ability with G4 and reduced the cytotoxicity of the Ir(Ⅲ) complex.Compared with other nucleic acid secondary structures, Ir-PDP produced a higher phosphorescence lifetime after interacting with G4. Ir-PDP was distributed in the cytoplasm of living cells, and two-photon phosphorescence lifetime imaging can detect the binding events of the probe in the cytoplasm. The addition of G4 binder PDS significantly regulated cytoplasmic phosphorescence lifetime. The project explored a new sensing pathway to observe the binding manners of probes in the cytoplasm through the phosphorescence lifetime of probes.

关 键 词：G-QUADRUPLEX Pyridostatin derivatives Iridium(Ⅲ)complex Phosphorescent probe Phosphorescence lifetime Cell imaging 

分 类 号：O657.3[理学—分析化学] Q2-33[理学—化学]