基于HPLC-ESI-QTOF-MS技术的金桂花降糖活性物质基础研究  

Research on hypoglycemic activity of Osmanthus fragrans var.thunbergii extract

作　　者：童俊[1] 刘红兵 刘颖伟 张帆[1] 孙丽娟[1] 陈勇[1] TONG Jun;LIU Hong-bing;LIU Ying-wei;ZHANG Fan;SUN Li-juan;CHEN Yong(National&Local Joint Engineering Research Center of High-throughput Drug Screening Technology,Key Laboratory of Biotechnology of Chinese Traditional Medicine,Health Science and Engineering,Hubei University,Wuhan 430062,China;State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,National Center for Magnetic Resonance in Wuhan,Innovation Academy for Precision Measurement Science and Technology,Chinese Academy of Sciences,Wuhan 430071,China)

机构地区：[1]湖北大学健康科学与工程学院,药物高通量筛选技术国家地方联合工程研究中心,中药生物技术湖北省重点实验室,湖北武汉430062 [2]中国科学院精密测量科学与技术创新研究院,波谱与原子分子物理国家重点实验室,武汉磁共振中心,湖北武汉430071

出　　处：《药学学报》2023年第3期750-759,共10页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(31400304);波谱与原子分子物理国家重点实验室开放项目(T151203)。

摘　　要：本文通过体外对α-葡萄糖苷酶活性、糖基化产物形成及抗氧化能力的影响,综合评价了金桂花水提物的降糖活性,并采用高效液相色谱-电喷雾-四极杆飞行时间串联质谱(HPLC-ESI-QTOF-MS)技术对金桂花水提物的主要化学成分进行了定性分析。体外药理活性研究表明,金桂花水提物对α-葡萄糖苷酶具有显著的抑制活性,抑制率(IC_(50)=2.11±0.26 mg·mL^(-1))与阿卡波糖相当(IC_(50)=2.88±0.32 mg·mL^(-1));同时具有一定的抑制糖基化产物和抗氧化能力。金桂花水提物中化合物质谱定性分析主要参考对照品毛蕊花糖苷质谱裂解规律和相关文献,鉴定出其中73个化合物,包括58个苯乙醇苷类化合物,8个咖啡酰奎宁酸类化合物,1个黄酮类化合物和几个植物中常见有机化合物。本研究初步推测61种为桂花中首次发现的化合物,其中8个目前尚无法鉴定结构的生物碱类化合物。研究结果为进一步阐明金桂花的降糖活性成分及制定科学的桂花质量控制标准提供了科学依据。This study aimed to assess the hypoglycemic activity,and in vitro inhibition ofα-glucosidase,inhibition of the advanced glycation end products(AGEs),and total antioxidant capacity were used to clarify its bioactivity.Furthermore,the potential hypoglycemic active chemical constituents in the aqueous extract of Osmanthus fragrans var.thunbergii flower were characterized using high performance liquid chromatographyelectrospray ionization-quadruple time-of-flight mass spectrometry(HPLC-ESI-QTOF-MS)method.The result showed that in vitro inhibition ofα-glucosidase of the extract(IC_(50)=2.11±0.26 mg·mL^(-1))were similar to acarbose(IC_(50)=2.88±0.32 mg·mL^(-1)),and it inhibited the AGEs formation and the total antioxidant capacity in a certain extent.Based on the MS fragmentation pathway analysis of reference chemical acteoside contained in this extract,and related references,73 constituents were tentatively identified from the aqueous extract of Osmanthus fragrans var.thunbergii flower,including 58 phenylethanoids,8 caffeoylquinic acids,1 flavonoid vicenin-2,and 6 common organic chemicals in plant.Furthermore,8 unknown alkaloids were characterized in this work.Among of these chemicals,61 phenylethanoids were supposed to be detected for the first time.In conclusion,this work disclosed the potential hypoglycemic active constituents of Osmanthus fragrans var.thunbergii flower.

关 键 词：金桂花 降糖 Α-葡萄糖苷酶 抗糖基化 抗氧化 

分 类 号：R284[医药卫生—中药学]