细菌内毒素检测方法的研究进展  

Research progress of bacterial endotoxin detection methods

作　　者：张媛 孙述学 ZHANG Yuan;SUN Shuxue(Beijing Chengda Tianhe Biotechnology Co.,Ltd.,Bejing 100176,China)

机构地区：[1]北京成大天和生物科技有限公司,北京100176

出　　处：《中国生物制品学杂志》2023年第3期368-372,378,共6页Chinese Journal of Biologicals

摘　　要：细菌内毒素作为一种强力的热原物质,极微量即可对人体健康产生严重影响,能引起发热、微循环障碍、内毒素血症、内毒素休克和弥散性血管内凝血等,严重者会造成死亡。因此,对医药产品进行内毒素检测至关重要。近年来,由于对鲎的过度捕捞以及环境的恶化等,我国鲎的数量急剧减少,现已将其列为国家二级保护动物,传统的鲎试验内毒素检测法将逐渐被替代。随着研究的不断深入,开发出一系列快速、灵敏、准确的新型内毒素检测方法。本文对各类内毒素检测方法(重点介绍了重组C因子法、生物传感器检测法等新型方法)、各自的优缺点及发展方向作一综述,以期使新型检测技术得到更广泛的开发和应用。As a powerful pyrogen substance,bacterial endotoxin in small amounts can cause many serious effects on human health and would cause fever,microcirculation disorders,endotoxemia,endotoxin shock,diffuse intravascular coagulation and even death.Therefore,it is very important to detect endotoxin in pharmaceutical products.In recent years,due to overfishing of horseshoe crab and environmental deterioration,the number of horseshoe crab in China is decreasing rapidly.It has been listed as the second-class protected animal in China,and the traditional endotoxin detection methods of limulus amoebocyte lysate will be replaced gradually.With the deepening of research,a series of rapid,sensitive and accurate methods for endotoxin detection have been developed.This paper reviews various endotoxin detection methods,focusing on their innovations such as recombinant factor C method and biosensor method,and elaborates their advantages,disadvantages and development trends with the hope that new detection technologies will be more widely developed and applied.

关 键 词：内毒素 鲎试剂 脂多糖 生物传感器 

分 类 号：R927.12[医药卫生—药学]