响应面法优化黑水虻幼虫蛋白肽的制备工艺  

Optimization of Preparation Process of Black Soldier Fly Larval Protein Peptides by Response Surface Method

作　　者：唐杏雨 理俊霞 王浩琳 郭建来[3] 刘昆 张红云 王心雨 韩广猛 李育鹏 张继臣 TANG Xingyu;LI Junxia;WANG Haolin;GUO Jianlai;LIU Kun;ZHANG Hongyun;WANG Xinyu;HAN Guangmeng;LI Yupeng;ZHANG Jichen(College of Animal Science and Technology,Henan University of Animal Husbandry and Economy,Zhengzhou He'nan 450046,China;College of Veterinary Medicine,Henan College of Animal Husbandry and Economy,Zhengzhou He'nan 450046,China;Henan University of Animal Husbandry and Economy,Henan Key Laboratory of Unconventional Feed Resources Innovative Utilization,Zhengzhou He'nan 450046,China)

机构地区：[1]河南牧业经济学院动物科技学院,河南郑州450046 [2]河南牧业经济学院动物医药学院,河南郑州450046 [3]河南牧业经济学院,河南省非常规饲料资源创新利用重点实验室,河南郑州450046

出　　处：《现代畜牧科技》2023年第4期6-11,共6页Modern Animal Husbandry Science & Technology

基　　金：河南省高等学校重点科研项目(22B230003);河南省科技攻关项目(222102110390);河南省科技攻关项目(222102110414)。

摘　　要：试验以黑水虻幼虫为主要原料,以多肽含量为指标,应用响应面法对菌酶协同发酵工艺条件进行优化。通过单因素试验对菌液接种量、菌液接种比例、加酶量、发酵温度和发酵时间5个主要工艺参数进行研究,在此基础上,通过响应面法优化菌酶协同发酵工艺,建立数学模型。结果表明,最佳菌酶协同发酵条件为植物乳杆菌和芽孢杆菌混合菌液接种量2.0%,发酵温度39℃,发酵时间62 h,在该条件下多肽含量为11.78%,实际值与理论值偏差较小,说明该模型能较好的预测黑水虻幼虫蛋白肽最佳制备工艺条件。Black soldierfly larva was used as the main raw material in this experiment.Response surface method was applied to optimize the process conditions of bacteria-enzyme cooperative fermentation by using polypeptide content as the index.First of all,five crucial process parameters,inoculated quantity,inoculated proportion of inoculated liquid,enzyme addition amount,fermentation temperature and fermentation time were studied by single factor experiment.Based on this,response surface method was used to optimize the cooperative fermentation process of bacteria and enzyme,and a mathematical model was established.The results showed that the optimal fermentation conditions were as followed:the inoculum quantity of Lactobacillus plantarum and Bacillus plantarum was 2.0%,fermentation temperature was 39℃,and fermentation time was 62 h.In this conditions,the polypeptide content was 11.78%.The deviation between the actual value and the theoretical value was small,which indicated that this model could predict the optimum preparation conditions of protein peptide of black soldierfly larva.

关 键 词：菌酶协同 黑水虻幼虫 响应面法 发酵工艺 

分 类 号：S816.7[农业科学—饲料科学]