2017-2021年江苏省临床分离耶尔森菌分子特征分析  

ly isolated Yersinia in Jiangsu Province from 2017 to 2021

作　　者：周璐[1] 程晓庆 段然[2] 仲慧[3] 祝雯雯[4] 鲍倡俊[1] 王鑫[2] 谈忠鸣[1] Zhou Lu;Cheng Xiaoqing;Duan Ran;Zhong Hui;Zhu Wenwen;Bao Changjun;Wang Xin;Tan Zhongming(NHC Key Laboratory of Enteric Pathogenic Microbiology,Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Mqjor Infectious Diseases,Jiangsu Provincial Center for Disease Control and Prevention,Nanjing 210009,China;National Institute for Infectious Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Clinical Laboratory,Dongtai Center for Disease Control and Prevention,Dongtai 224299,China;Clinical Laboratory,Xuzhou Center for Disease Control and Prevention,Xuzhou 221002,China)

机构地区：[1]国家卫生健康委员会肠道病原微生物重点实验室、江苏省新发突发重大传染病病原微生物重点实验室、江苏省疾病预防控制中心,南京210009 [2]中国疾病预防控制中心传染病预防控制所,北京102206 [3]东台市疾病预防控制中心检验科,东台224299 [4]徐州市疾病预防控制中心检验科,徐州221002

出　　处：《中华地方病学杂志》2023年第3期190-195,共6页Chinese Journal of Endemiology

基　　金：国家科技重大专项(2018ZX10713-003-002、2018ZX10713-001-002);江苏省卫生健康委科研重点项目(ZD2021037)。

摘　　要：目的:了解江苏省腹泻患者临床分离耶尔森菌的分布特点及分子特征。方法:2017-2021年,在小肠结肠炎耶尔森菌国家级主动监测点江苏省徐州市铜山区和盐城市东台市,采集腹泻患者粪便标本,进行耶尔森菌菌株分离;同时,收集全省哨点医院疑似耶尔森菌菌株。提取分离菌株DNA进行全基因组重测序,并上传至EnteroBase数据库进行耶尔森菌种型鉴定,原始数据经清洁和处理后进行16S核糖体RNA(16S rRNA)基因多态性分析;通过美国国家生物技术信息中心(NCBI)和病原菌毒力因子数据库(VFDB)扫描5种毒力基因(ail、ystA、ystB、yadA、virF)携带情况,同时构建K-mer树并进行基因组特征分析。结果:2017-2021年,共采集腹泻患者粪便标本2 058份,经分离鉴定,得到57株耶尔森菌;同时,通过哨点医院收集到2株耶尔森菌。经EnteroBase数据库比对,51株菌为小肠结肠炎耶尔森菌,4株菌为 Yersinia proxima,阿利克西奇耶尔森菌、马赛耶尔森菌、中间耶尔森菌及 Yersinia canariae各1株。16S rRNA基因多态性分析显示,全部菌株被聚类为3个大组,可区分小肠结肠炎耶尔森菌和其他耶尔森菌。51株小肠结肠炎耶尔森菌中,49株为毒力基因Ⅲ型(ail-、ystA-、ystB+、yadA-、virF-),2株为毒力基因Ⅱ型(ail+、ystA+、ystB-、yadA-、 virF-);8株其他耶尔森菌均为毒力基因Ⅳ型(ail-、ystA-、ystB-、yadA-、virF-)。K-mer分析可区分小肠结肠炎耶尔森菌和其他耶尔森菌,JS-XZ-2020001菌株与其他小肠结肠炎耶尔森菌距离较远,血清型为O8的小肠结肠炎耶尔森菌分布较集中。 结论:江苏省腹泻患者临床分离耶尔森菌以小肠结肠炎耶尔森菌为主,其他耶尔森菌少量并存,同时发现了 Yersinia proxima和 Yersinia canariae两个耶尔森菌新种型。小肠结肠炎耶尔森菌的毒力基因携带以Ⅲ型为主。16S rRNA基因多态性分析和K-mer分析均可有效区分小肠结肠炎耶尔森菌和其他耶尔�Objective To investigate the distribution and molecular characteristics of Yersinia isolated from diarrhea patients in Jiangsu Province.Methods From 2017 to 2021,the stool samples of diarrhea patients were collected in Tongshan District of Xuzhou City and Dongtai City of Yancheng City,Jiangsu Province,where the national active monitoring sites of Yersinia enterocolitica,then Yersinia was isolated;meanwhile,suspected Yersinia strains were collected from sentinel hospitals in the province.The DNA of isolated strains was extracted for whole genome resequencing,and the data were uploaded to the EnteroBase database for Yersinia species identification;the original data were cleaned and processed for 16S ribosomal RNA(16S rRNA)gene polymorphism analysis.Five virulence genes(ail,ystA,ystB,yadA,virF)were scanned through the National Center for Biotechnology Information(NCBI)and Pathogen Virulence Factor Database(VFDB),and K-mer Tree was constructed and genomic characteristics were analyzed.Results From 2017 to 2021,a total of 2058 stool samples from diarrhea patients were collected,and 57 strains of Yersinia were isolated and identified;meanwhile,two Yersinia strains were collected from the sentinel hospital.Compared with EnteroBase database,51 strains were identified as Yersinia enterocolitica,4 strains as Yersinia proxima,1 strain each as Yersinia aleksiciae,Yersinia massiliensis,Yersinia intermedia and Yersinia canariae.The 16S rRNA gene polymorphism analysis showed that all strains were clustered into 3 groups,which could distinguish Yersinia enterocolitica from other Yersinia.Among the 51 strains of Yersinia enterocolitica,49 strains were virulence genotype Ⅲ(ail-,ystA-,ystB+,yadA-,virF-),two strains were virulence genotype Ⅱ(ail+,ystA+,ystB-,yadA-,virF-);and 8 other Yersinia strains were virulence genotype Ⅳ(ail-,ystA-,ystB-,yadA-,virF-).K-mer analysis could distinguish Yersinia enterocolitica from other Yersinia,JS-XZ-2020001 strain was far away from other Yersinia enterocolitica isolates,and serotype 08 stra

关 键 词：耶尔森菌属 全基因组重测序 毒力基因 16S核糖体RNA基因多态性 Yersinia proxima Yersinia canariae 

分 类 号：R574.62[医药卫生—消化系统] R446.5[医药卫生—内科学]