微纳米磷酸钛涂层促进钛植入体骨结合的实验研究  

Micro-nano titanium phosphate coating promoted osseointegration of titanium material

作　　者：孙祎昕 祝颂松[1] 姜楠[1] SUN Yi-xin;ZHU Songsong;JIANG Nan(State Key Laboratory of Oral Diseases,National Clinical Research Center for Oral Diseases,Department of Orthognathic and Temporomandibular Joint Surgery,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,Sichuan Province,China)

机构地区：[1]口腔疾病研究国家重点实验室,国家口腔疾病临床医学研究中心,四川大学华西口腔医院正颌及关节外科,四川成都610041

出　　处：《中国口腔颌面外科杂志》2023年第2期112-118,共7页China Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金(81901026)。

摘　　要：目的:探讨在钛(Ti)植入体表面制备微纳米磷酸钛涂层以促进骨结合的效果。方法:通过特定条件的磷酸水热反应,制备出微纳米磷酸钛涂层的钛植入体,即微/纳米棒状涂层钛(micro/nanorod Ti,P-R-Ti),对照组为未处理的光滑纯钛(cp-Ti)。对材料进行成分、形貌、亲水性等表征分析,并在体外条件下测试细胞增殖活力,分析与细胞黏附、分化相关蛋白及基因表达的变化,探讨P-R-Ti对大鼠骨髓间充质干细胞(BMSCs)生物学行为的影响,评估P-R-Ti植入大鼠胫骨12周后对骨结合的影响,并对其生物力学强度进行测试。采用SPSS 21.0软件包对数据进行统计学分析。结果:材料表征表明,P-R-Ti表面具有密集的微纳米级棒状结构。体外实验表明,P-R-Ti组黏附相关蛋白及mRNA表达上调,细胞活力显著增强,ALP活性、Runx-2、Col-1、OPN、OCN mRNA的表达水平显著高于cp-Ti组。体内实验表明,P-R-Ti组骨面积比、骨骼与植入物之间的接触显著高于cp-Ti组,其最大推出力可达(179.3±11.3)N,极限抗剪切强度可达(19.25±1.85)N/mm2。结论:P-R-Ti可有效促进钛植入体的骨结合能力,有望作为一种良好的钛表面改性方法应用于临床。PURPOSE:To fabricate micro-nano titanium phosphate coating on the surface of titanium(Ti)material to promote osseointegration.METHODS:Through the hydrothermal reaction of phosphoric acid under specific conditions,a Ti sheet/cylinder with a coating of micro-nano titanium phosphate,namely micro/nanorod Ti(P-R-Ti),was prepared.The control group was untreated smooth pure Ti(cp-Ti).The materials were characterized and analyzed in terms of composi-tion,morphology,hydrophilicity,etc.,and the cell proliferation activity was tested in vitro.The changes in protein and gene expression related to cell adhesion and differentiation were analyzed to explore the effect of P-R-Ti on the biological behavior of rat bone marrow mesenchymal stem cells(BMSCs).At the same time,the effects of P-R-Ti on bone union were evaluated 12 weeks after implantation into rat tibia,and its biomechanical strength was tested.SPSS 21.0 software package was used for data analysis.RESULTS:The material characterization showed that the surface of P-R-Ti had a dense micro nano rod structure.In vitro experiments showed that the expression of adhesion related proteins and mRNA in P-R-Ti group was up-regulated,and the cell viability was significantly enhanced.The expression levels of ALP activity,Runx-2,Col-1,OPN,OCN mRNA in P-R-Ti group were significantly higher than those in cp Ti group.In vivo experi-ments showed that the bone area ratio and the contact between bone and implant in P-R-Ti group were significantly high-er than those in cp-Ti group.The maximum pushing force was(179.3±11.3)N,and the ultimate shear strength was(19.25±1.85)N/mm2.CONCLUSIONS:P-R-Ti can effectively promote the osseointegration of titanium material.It is expected to be used as an excellent method of titanium surface modification for clinical application.

关 键 词：微/纳米多级界面 骨结合 植入体 磷酸钛 

分 类 号：R783.1[医药卫生—口腔医学]