经络辨证逆针灸对二甲基苯蒽诱导的大鼠乳腺肿瘤发生过程的影响  

Effect of Preventive Acupuncture Based on Meridian Syndrome Differentiation on DMBA-induced Mammary Tumorigenesisin Rats

作　　者：董芹作 李襄 杨隽 陈颖 夏春林 赵荣 DONG Qinzuo;LI Xiang;YANG Jun;CHEN Yin;XIA Chunlin;ZHAO Rong(Yunnan University of Chinese Medicine,Kunming 650500,Yunnan,China)

机构地区：[1]云南中医药大学,云南昆明650500

出　　处：《辽宁中医杂志》2023年第2期181-185,I0005,I0006,共7页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81760896);云南省教育厅科学研究基金项目(2020J0294)。

摘　　要：目的 观察经络辨证逆针灸对二甲基苯蒽[7,12-dimethylbenz-(a)anthranene, DMBA]诱导的乳腺肿瘤模型大鼠肿瘤发生过程的影响。方法 选取8周龄清洁级雌性SD未孕大鼠64只,随机分为空白对照组、模型组、他莫昔芬组和逆针灸组,每组各16只。采用DMBA单次灌胃(100 mg/kg)诱导自发性乳腺癌模型,空白对照组给予等量芝麻油灌胃。于造模后1周对治疗组进行干预,他莫昔芬组3 mg/kg灌胃,1次/d,持续16周;逆针灸组针刺“足三里、膻中、三阴交”,隔天1次,每次20 min,每周3次,干预16周。应用HE染色评估大鼠乳腺组织形态学变化;应用免疫组织化学法进行CK5/6、p63检测,观察其在不同类型病变乳腺组织中的分布及表达水平。结果 HE染色以及免疫组化染色CK5/6和p63表达结果显示,DMBA诱导的乳腺肿瘤模型大鼠肿瘤的发生发展途径为:正常→一般增生→非典型增生→原位癌→浸润性癌;对不同实验组乳腺组织不同类型病变的分布进行统计学分析,结果提示各组间组织病理学分布具有统计学意义(P<0.05)。结论 经络辨证理论指导下的逆针灸能够抑制DMBA诱导乳腺自发性肿瘤模型大鼠病变进展,有望应用于乳腺癌的临床防治。Objective To observe the effect of preventive acupuncture based on meridian syndrome differentiation on tumorigenesis induced by 7,12-dimethylbenz-(a)anthranene(DMBA)in rats with mammary tumor.Methods Sixty-four 8-week-old female SD non-pregnant rats were randomly divided into blank control group, model group,tamoxifen group and preventive acupuncture group, with 16 rats in each group.Spontaneous mammary cancer model was induced by intragastric administration of DMBA(100 m g/kg),and the blank control group was intragastrically administered with the same amount of sesame oil.The treatment group was intervened one week after modeling, and the tamoxifen group was intragastrically administered with tamoxifen(3 m g/kg) once a day for 16 weeks.The preventive acupuncture group was treated with acupuncture at “Zusanli(ST36),Danzhong(RN17)and Sanyinjiao(SP6)” every other day for 20 minutes, three times a week, totally for 16 weeks.HE staining was used to assess the histomorphological changes of rat mammary glands.Immunohistochemistry was used to detect CK5/6 and p63 to observe their distribution and expression levels in different types of lesion mammary tissues.Results The results of HE staining and immunohistochemical staining of CK5/6 and p63 expression showed that the occurrence and development pathways of tumors in DMBA-induced mammary tumor model rats were: normal→general hyperplasia→atypical hyperplasia→carcinoma in situ→invasive carcinoma.The distribution of different types of lesions in mammarytissues of different experimental groups was statistically analyzed, and the results suggested that there was a statistically significant difference in histopathological distribution among the groups(P<0.05).Conclusion Preventive acupuncture guided by the theory of meridian syndrome differentiation can inhibit the progression of DMBA-induced spontaneous mammary tumor model in rats, which is expected to be applied in the clinical prevention and treatment of breast cancer.

关 键 词：逆针灸 乳腺肿瘤发生过程 不典型增生 原位癌 浸润性癌 

分 类 号：R737.9[医药卫生—肿瘤]