基于形态分类和花粉粒亚显微结构特征的金银花农家品种鉴定  

Identification of Lonicera japonica varieties based on morphological classification and submicroscopic structure characteristics of pollen grains

作　　者：李晓娟 卢恒 刘伟[1,2] 李圣波 王晓 LI Xiaojuan;LU Heng;LIU Wei;LI Shengbo;WANG Xiao(Shandong Analysis and Test Center,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China;School of pharmacy,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China;Shandong Xiandai University,Jinan 250104,China;Shandong Yate Ecological Technology Co.,Ltd.,Linyi 276017,China)

机构地区：[1]齐鲁工业大学(山东省科学院)、山东省分析测试中心,山东济南250014 [2]齐鲁工业大学(山东省科学院)药学院,山东济南250014 [3]山东现代学院,山东济南250104 [4]山东亚特生态技术股份有限公司,山东临沂276017

出　　处：《山东科学》2023年第2期1-7,共7页Shandong Science

基　　金：国家科技基础资源调查专项(2018FY100702);山东省自然科学基金(ZR2020QC021);财政部和农业农村部国家现代农业产业技术体系项目(CARS-21);全国中药资源普查项目(2019-1024)。

摘　　要：通过研究花粉粒亚显微结构特征,为评价忍冬种质资源、品种分类鉴定及优良品种选育提供依据。采用光学显微镜和扫描电镜对金银花农家品种植株、茎、叶、花和花粉及外壁雕纹进行形态分类鉴定,数据采用SPSS进行统计分析和评价。8个主流农家品种形态性状特征及花粉粒形状、萌发孔及外壁刺状雕纹等均有一定差异。四季花植株杆冠明显,北花1号灌丛状,金银花24号花蕾疏被柔毛,花粉粒呈球形,外壁具极密的刺状雕纹;北花1号和四季花的花粉粒呈三角状球形,外壁具密粗刺状雕纹;大毛花茎枝及花蕾密被长柔毛,金银花16号花蕾密被腺毛和针状短毛,金银花1号、金银花15号及金银花23号花粉粒呈钝三角状球形或类球形,外壁具稀疏刺状雕纹。金银花农家品种的花粉粒形态特征为品种分类和鉴定提供了孢粉学依据。This study aimed to provide a basis for the evaluation of germplasm resources,variety classification and identification,and selection of excellent varieties for Lonicera japonica based on submicroscopic structure characteristics of pollen grains.Optical microscope and scanning electron microscope were used to classify and identify the plants,stems,leaves,flowers,pollens,and outer ornamentations of L.japonica varieties.The data were analyzed using statistical product and service solutions(SPSS).There were certain differences in the morphological traits,pollen shapes,germinal apertures,and outer ornamentations of the eight L.japonica varieties.The stem and crown of the Sijihua plants were obvious,and the Beihua No.1 plants were bush-like.The buds of L.japonica No.24 were sparsely pubescent.The pollen grains were spheres,with dense thorn-like ornamentations on the outer wall.The pollen grains of Beihua No.1 and Sijihua were triangular spheres,with dense,thick,thorn-like ornamentations on the outer wall.The stems,branches,and buds of Damaohua had dense,long pubescence.The buds of L.japonica No.16 were densely covered,with glandular hairs and acicular hairs.The pollen grains of L.japonica No.1,L.japonica No.15,and L.japonica No.23 were blunt triangular spheres or spheroids,with sparse,thorn-like ornamentations on the outer wall.The morphological characteristics of the pollen grains of L.japonica varieties provide a palynological basis for variety classification and identification.

关 键 词：忍冬 品种 花粉粒 孔沟 扫描电镜 

分 类 号：R284.1[医药卫生—中药学]