竹红菌素中试提纯工艺及抑菌活性研究  

Study on pilot-scale purification process and antibacterial activity of hypocrellin

作　　者：孙博通 李洪亮 韩如冰 王英 张思晨 SUN Botong;LI Hongliang;HAN Rubing;WANG Ying;ZHANG Sichen(Science and Technology Service Platform(Overseas Chinese Pioneer Park),Qilu University of Technology(Shandong Academy of Sciences),Jinan 250100,China;Shandong SME Productivity Promotion Center,Jinan 250000,China;Zibo Municipal Hospital,Zibo 255400,China)

机构地区：[1]齐鲁工业大学(山东省科学院)、山东省科学院高新技术产业(中试)基地(山东省科学院留学人员创业园),山东济南250000 [2]山东中小企业生产力促进中心,山东济南250000 [3]淄博市立医院,山东淄博254000

出　　处：《山东科学》2023年第2期8-15,共8页Shandong Science

基　　金：“一带一路”创新人才交流外国专家项目(DL2021023002L)。

摘　　要：为了研究竹红菌素的中试提纯工艺及抑菌效果,在中试生产中,通过不同溶剂、提取方法提取竹红菌素,研究盐析法、降温法和超滤法对竹红菌素的纯化效果。结果表明使用气流粉碎法竹红菌素提取量可达21.4 mg/g,超滤法纯化后,竹红菌素的纯度达到90%以上。对竹红菌素的抑菌活性分析发现,其热稳定性良好,并且对大肠埃希氏菌和白色念珠菌均有抑制作用。竹红菌素具有天然抗菌和良好的热稳定性,易提取纯化,可作为一种抑菌药物应用到医药等领域。To study the pilot-scale purification process and bacteriostatic effect of hypocrellin during the pilot-scale production,we extracted hypocrellin using different solvents and extraction methods.Then,we investigated the purification effects of the hypocrellin using salting-out,physical cooling,and ultrafiltration methods.The results showed that the amount of hypocrellin extracted using the jet pulverization method could reach up to 21.4 mg/g,and its density could exceed 90%after its purification via ultrafiltration.The analysis of the bacteriostatic activity of hypocrellin showed that it had good thermal stability and inhibitory effects against Escherichia coli and Candida albicans.We found that hypocrellin has natural antibacterial properties and good thermal stability,is easy to extract and purify,and can be used as a bacteriostatic drug in medicine and other applications.

关 键 词：竹红菌素 提取 纯化 稳定性 抑菌 

分 类 号：R284.2[医药卫生—中药学]