针刺对脑梗死大鼠缺血脑组织血管新生相关因子表达的影响  

Effects of acupuncture on angiogenesis-associated factor expression in ischemic brain tissue following cerebral infarction in rats

作　　者：孙雅洁 李晶[1,2] Rainer Georgi Bernhard Kolberg 杨丽红 Yajie Sun;Jing Li;Rainer Georgi;Bernhard Kolberg;Lihong Yang(Tianjin University of Traditional Chinese Medicine First Affiliated Hospital,Tianjin,China;National Center for Chinese Medicine Acupuncture Clinical Medicine Research,Tianjin,China;Department of Chemical Biology,Max Planck Institute for Medical Research,Heidelberg,Germany;Department of Internal Medicine,Mannheim Medical School of Heidelberg University,Mannheim,Germany)

机构地区：[1]天津中医药大学第一附属医院,天津300193 [2]国家中医针灸临床医学研究中心,天津300193 [3]Department of Chemical Biology,Max Planck Institute for Medical Research,Germany [4]Department of Internal Medicine,Mannheim Medical School of Heidelberg University,Germany

出　　处：《Acupuncture and Herbal Medicine》2023年第1期46-54,共9页针灸和草药（英文）

基　　金：National Natural Science Foundation of China(General Program)(No.81674056);Natural Science Foundation of Tianjin(No.18JCYBJC94200).

摘　　要：目的:动态研究脑梗死后血管新生相关因子的变化规律和针刺干预效应,从促进血管新生角度深入揭示针刺治疗脑梗死的分子机制。方法:以MCAO模型大鼠为研究对象,分为针刺组、模型组和空白组。观察脑梗死后神经功能缺损程度改变,同时采用免疫荧光双标记法观察缺血中心及边缘区的血管新生情况,应用RT-PCR和Western blot法检测脑梗死后缺穴区域血管新生相关因子Ang-1、Ang-2、PDGF-B和bFGF mRNA及蛋白变化规律和电针干预效应。结果:电针组各时相NSS评分均小于同时相模型组。MCAO后9h~7d时电针组血管内皮细胞增殖高于同时相模型组。电针组Ang-1、Ang-2 mRNA及蛋白表达水平明显高于模型组。电针组PDGF-B mRNA在3h~6h、3d~12d时明显高于模型组,蛋白水平在6h、3d~12d时明显高于模型组。针刺组bFGF mRNA在24h~12d、蛋白水平在3d~12d时明显高于模型组。结论:电针水沟穴能明显改善MCAO大鼠神经功能症状;能够促进MCAO模型大鼠梗死区周围血管内皮细胞增殖,并将血管内皮细胞增殖出现时间显著提前,其可能机制为电针水沟穴可上调血管新生相关因子的表达,并使表达时相前移,从而促进血管新生,对改善脑梗死的预后起到了明显的积极作用。Background:We aimed to investigate changes into the levels of angiogenesis-associated factors following cerebral infarction and acupuncture intervention and reveal the underlying molecular mechanisms involved in promoting angiogenesis.Methods:Model rats with middle cerebral artery occlusion(MCAO)were randomized into electroacupuncture(EA),model control(MC),and blank control(control)groups.Changes in the degree of neurological impairment following cerebral infarction and angiogenesis in the ischemic center and peripheral area were observed using immunofluorescence double-labeling.Reverse transcription-polymerase chain reaction(RT-PCR)and western blotting were used to detect changes in the Ang-1,Ang-2,PDGF-B,and bFGF levels.Moreover,the effects of EA intervention were evaluated.Results:The neurological severity score of each phase in the EA group was lower than that into the simultaneous phase in the MC group.The proliferation of vascular endothelial cells in the EA group was higher than that in the MC group at 12 hours to 7 days.The Ang-1 and Ang-2 mRNA and protein levels in the EA group were significantly higher than those in the MC group.PDGF-B levels in the EA group were significantly higher than those in the MC group at 3 to 6 hours and 3 to 12 days,and protein levels were high at 6 hours and 3 to 12 days.bFGF mRNA levels at 24 hours to 12 days and bFGF protein at 3 to 12 days were significantly elevated in the EA group than those in the MC group.Conclusions:EA at Shui Gou(DU 26)significantly improved the neurological symptoms of MCAO rats,promoted vascular endothelial cell proliferative activity around the infarct area,significantly advanced the time of proliferation of vascular endothelial cells,and upregulated the expression of angiogenesis-related factors,thereby promoting angiogenesis.Thus,EA may significantly improve the prognosis of cerebral infarction.

关 键 词：人中穴 脑梗死 电针 血管新生 

分 类 号：R73[医药卫生—肿瘤]