猕猴桃MYB家族成员鉴定及其低温表达分析  

Identification and Low Temperature Expression Analysis of MYB Transcription Factor Family in Kiwifruit

作　　者：毛可欣 安淼[1] 王海荣[1] 王世金 吕巍[2] 郭盈添 李健 李国田[1] MAO Kexin;AN Miao;WANG Hairong;WANG Shijin;LÜWei;GUO Yingtian;LI Jian;LI Guotian(Shandong Institute of Pomology,Tai’an,Shandong 271000,China;Shandong Agricultural University,Tai’an,Shandong 271018,China;North Kiwifruit(Rizhao)Technology Research Institute,Rizhao,Shandong 276800,China)

机构地区：[1]山东省果树研究所,山东泰安271000 [2]山东农业大学,山东泰安271018 [3]北方奇异果(日照)技术研究院,山东日照276800

出　　处：《园艺学报》2023年第3期534-548,共15页Acta Horticulturae Sinica

基　　金：中央引导地方科技发展专项(YDZX20193700004330);山东省农业科学院创新工程项目(CXGC2021A31,CXGC2021B34)。

摘　　要：通过对‘红阳’猕猴桃基因组鉴定得到277个MYB家族成员并将其分为12个亚家族(S1~S12),其中多数序列属于R2R3-MYB,其次是MYB相关蛋白,仅鉴定到1个4R-MYB。S11和S12亚家族在进化上处于较早的位置,结构较为多样化,而S1和S2在进化上出现较晚。顺式作用元件分析显示,该家族主要含水杨酸、赤霉素和茉莉酸甲酯等相关植物激素元件,还有部分参与昼夜节律、低温、细胞周期等相关元件。密码子偏好性分析显示,猕猴桃MYB家族有3个高频密码子(UUG、AGA和AGG),基因的密码子偏好使用A/T,第3位碱基偏好更强。通过顺式作用元件分析和4个猕猴桃品种越冬期转录组数据分析筛选了9个可能参与低温胁迫响应MYB基因,对其在低温胁迫下的软枣猕猴桃‘龙成2号’中进行检测验证,确认这些基因参与猕猴桃对低温的响应。In this paper,277 MYB family genes were identified through the genome of Actinidia chinensis‘Hongyang’.The MYBs were classified into 12 subclasses(S1-S12),most of the sequences belong to R2R3-MYB,followed by MYB-related proteins,and only one 4R-MYB identified.S11 and S12 were at an earlier evolutionary position with more diverse response structures,never the less S1 and S2 were at a later evolutionary position.The cis-acting element analysis showed that the main related phytohormones elements of this family are salicylic acid,gibberellin,and methyl jasmonate,further,there are elements partly involved in the regulation of circadian rhythm,low temperature,and cell cycle,etc.Codon preference analysis showed that the kiwifruit MYB family had three high-frequency codons(UUG,AGA and AGG).The codon preference of the gene was A/T,and the third base preference was stronger.Through the cis-acting elements and transcriptional data of four varieties kiwifruit in winter,nine MYB genes which possible response to cold stress.It was confirmed that these genes were involved in the response to low temperature by detecting and verifying in Actinidia arguta‘Longcheng 2’.

关 键 词：猕猴桃 MYB家族 密码子偏好性 低温胁迫 表达 

分 类 号：S663.4[农业科学—果树学]