水产养殖水体附着生物对磺胺甲恶唑胁迫响应及其降解效应  

RESPONSE OF PERIPHYTON IN AQUACULTURE WATER TO SULFAMETHOXAZOLE STRESS AND ITS DEGRADATION

作　　者：张心怡 闵奋力 张淑娴 张浩坤 李竹栖 彭雪 吴振斌[1,4] 刘碧云 ZHANG Xin-Yi;MIN Fen-Li;ZHANG Shu-Xian;ZHANG Hao-Kun;LI Zhu-Xi;PENG Xue;WU Zhen-Bin;LIU Bi-Yun(State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;University of Chinese Academy of Sciences,Beijing 100049,China;Changjiang Institute of Survey,Planning,Design and Research,Wuhan 430071,China;School of Environmental Studies,China University of Geosciences,Wuhan 430074,China)

机构地区：[1]中国科学院水生生物研究所,武汉430072 [2]中国科学院大学,北京101400 [3]长江勘测规划设计研究院有限责任公司,武汉430071 [4]中国地质大学(武汉),武汉430074

出　　处：《水生生物学报》2023年第5期739-746,共8页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(32171632和31830013);湖北省重点研发计划(2020BCA073)资助。

摘　　要：研究通过模拟养殖水体,探究附着生物对磺胺甲恶唑(SMX)的胁迫响应及其对SMX的降解途径。结果表明,在5和10 mg/L SMX胁迫下,附着生物胞外聚合物(EPS)分泌较对照组增加31.3%和21.5%,抗氧化能力降低20.6%和31.8%,但生物量的积累没有显著差异。SMX使附着生物抗氧化系统受到损伤,并刺激其分泌更多胞外聚合物以提高耐受性。去除实验结果表明,附着生物会促进SMX的消减,1 g/L附着生物在第14天对SMX的去除率为25.10%。主要降解中间产物有N4-乙酰基-磺胺甲恶唑(TP295)、对苯醌亚胺(TP107)、去砜基磺胺甲恶唑(TP189)和去氨基磺胺甲恶唑(TP238),键的断裂和苯环上氨基反应是SMX降解的两种主要途径。研究结果对评估SMX在养殖水体中的生态风险和利用附着生物去除SMX提供技术支撑。The extensive use and accumulation of sulfamethoxazole(SMX)in the aquaculture contributed to its residues in aquaculture water,which may cause various negative impacts on the aquatic ecological environment.Furthermore,periphyton is a common micro-ecosystem in water bodies composed of autotrophs and heterotrophs,including algae,fungi,bacteria,protozoa,metazoans,extracellular polymers(EPS)and debris,etc.Nevertheless,there is a lack of research on the impact of SMX on the growth and development of periphyton and its purification function in aquaculture water.In this survey,the effect of SMX on periphyton was researched in simulating aquaculture water,and the response of periphyton to SMX was explored through the biomass and total antioxidant capacity of the periphyton.The removal effect and the main degradation intermediates of SMX by periphyton were investigated by high-performance liquid chromatography(HPLC)and ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometry(UPLC-QTOF-MS).This research demonstrated that under 5 and 10 mg/L SMX,the secretion of extracellular polymer(EPS)of the periphyton increased by 31.3%and 21.5%compared with the control group,and the antioxidant capacity diminished by 20.6%and 31.8%,but there was no significant difference in biomass accumulation.It showed that SMX decreased the total antioxidant capacity of the mature periphyton,their oxidative systems were damaged,and more extracellular polymers were secreted to enhance tolerance to SMX.The results of the removal experiment indicated that the periphyton would promote the reduction of SMX,and the removal rate of SMX at 1 g/L was 25.10%on the 14th day.It is supposed that SMX was used by periphyton as a carbon source to promote biodegradation and shorten the half-life of SMX degradation.Thus,periphyton had a certain potential for SMX bioremediation in aquaculture water.The main routes of SMX biodegradation by the periphyton are the cleavage and activation of the sulfonamide group(N-S bond)and the amine group(-NH

关 键 词：磺胺甲恶唑 附着生物 生理响应 生物降解 

分 类 号：X171.5[环境科学与工程—环境科学]