微小RNA-433-3p靶向同源异形盒基因A1抑制胃癌MGC-803细胞增殖、侵袭的研究  

Experimental study of miR-433-3p targeting HOXA1 to inhibit the proliferation and invasion of gastric MGC-803 cancer cells

作　　者：赵轶峰[1] 李明霞[2] 张超 胡晓敏 王雄 赵铁军 Zhao Yifeng;Li Mingxia;Zhang Chao;Hu Xiaomin;Wang Xiong;Zhao Tiejun(Department of Gastrointestinal Tumor Surgery,the First Hospital Affiliated to Hebei North University,Zhangjiakou 075000,Hebei,China;Department of Internal Medicine and Metabolic Diseases,the First Hospital Affiliated to Hebei North University,Zhangjiakou 075000,Hebei,China;Department of Interventional,the First Hospital Affiliated to Hebei North University,Zhangjiakou 075000,Hebei,China)

机构地区：[1]河北北方学院附属第一医院胃肠肿瘤外科,河北张家口075000 [2]河北北方学院附属第一医院内分泌与代谢病科,河北张家口075000 [3]河北北方学院附属第一医院介入科,河北张家口075000

出　　处：《消化肿瘤杂志（电子版）》2023年第1期58-61,66,共5页Journal of Digestive Oncology(Electronic Version)

摘　　要：目的探讨微小RNA-433-3p(miR-433-3p)靶向同源异形盒基因A1(HOXA1)对胃癌MGC-803细胞增殖、侵袭的影响。方法体外培养人胃癌MGC-803细胞,分为对照组(正常培养,不进行任何处理)、si-NC组(转染miR-433-3p siRNA阴性对照)、si-miR-433-3p组(转染miR-433-3p siRNA)、mimic-NC组(转染miR-433-3p mimic阴性对照)和miR-433-3p mimic组(转染miR-433-3p mimic)。荧光定量聚合酶链反应(PCR)法检测MGC-803细胞中miR-433-3p及HOXA1 mRNA表达;细胞计数法、Transwell法分别检测MGC-803细胞增殖、侵袭;双荧光素酶报告基因试验检测miR-433-3p与HOXA1的靶向关系。结果与对照组和si-NC组相比,si-miR-433-3p组MGC-803细胞中miR-433-3p表达降低(P<0.05),HOXA1 mRNA表达、细胞增殖率、侵袭细胞数升高(P<0.05)。与对照组和mimic-NC组相比,miR-433-3p mimic组MGC-803细胞中miR-433-3p表达升高(P<0.05),HOXA1 mRNA表达、细胞增殖率、侵袭细胞数降低(P<0.05)。TargetScan网站预测及双荧光素酶报告基因试验结果显示,miR-433-3p与HOXA1在MGC-803细胞中存在靶向关系。结论miR-433-3p能靶向调控HOXA1抑制胃癌MGC-803细胞的增殖、侵袭过程。To investigate the effects of microRNA-433-3p(miR-433-3p)targeting homeobox gene A1(HOXA1)on the proliferation and invasion of gastric cancer MGC-803 cells.Method Human gastric cancer cells MGC-803 were cultured in vitro and divided into control group,si-NC group,simiR-433-3p group,mimic-NC group,and miR-433-3p mimic group.Real-time fluorescent quantitative PCR method was used to detect the expression of miR-433-3p and HOXA1 mRNA in MGC-803 cells.Cell counting,Transwell method were used to detect the proliferation,invasion of MGC-803 cells.The dual luciferase reporter gene experiment was used to detect the targeting relationship between miR-433-3p and HOXA1.Result Compared with the control group and si-NC group,the expression level of miR-433-3p in MGC-803 cells in the si-miR-433-3p group reduced(P<0.05),the HOXA1 mRNA expression level,cell proliferation rate,number of invasive cells increased(P<0.05).Compared with the control group and mimic-NC group,the expression level of miR-433-3p in MGC-803 cells in the miR-433-3p mimic group increased significantly(P<0.05),the HOXA1 mRNA expression level,cell proliferation rate,number of invasive cells reduced(P<0.05).TargetScan website prediction and dual luciferase reporter gene experiment results showed that miR-433-3p and HOXA1 had a targeting relationship in MGC-803 cells.Conclusion miR-433-3p can target HOXA1 to inhibit the proliferation and invasion of gastric cancer MGC-803 cells.

关 键 词：微小RNA-433-3p 同源异形盒基因A1 胃癌MGC-803细胞 增殖 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]