三种多酚类化合物对模拟间断性高原低氧暴露小鼠肠道菌群的影响  

Effects of three Polyphenolic compounds on the intestinal flora of mice exposed simulated intermittent plateau hypoxia

作　　者：潘存瑶 张宝怡 梁兰兰 刘辉[2] 郭长江 陈照立 王新兴 PAN Cun-yao;ZHANG Bao-yi;LIANG Lan-lan;LIU Hui;GUO Chang-jiang;CHEN Zhao-li;WANG Xin-xing(Institute of Environmental and Operational Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Tianjin 300050;School of Public Health,Lanzhou University,Lanzhou 730000;Chengdu Center for Disease Control and Prevention,Chengdu 610000,China)

机构地区：[1]军事科学院军事医学研究院环境医学与作业医学研究所,天津300050 [2]兰州大学公共卫生学院,甘肃兰州730000 [3]成都市疾病预防控制中心,四川成都610000

出　　处：《中国应用生理学杂志》2022年第5期392-397,共6页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金面上项目(31971106,18CXZ044)。

摘　　要：目的:探索三种多酚类化合物对高原低氧暴露小鼠肠道菌群的保护作用。方法:将60只雄性Balb/c小鼠随机分为平原对照组、模拟高原低氧组、高原原花青素组、高原槲皮素组、高原白藜芦醇组,每组12只。原花青素、槲皮素、白藜芦醇分别以50 mg/kg,100 mg/kg,20 mg/kg的剂量进行灌胃,三种药物干预且高原模拟舱模拟海拔6000 m暴露30 d后,收集血清进行二胺氧化酶(DAO)检测,收集小鼠无菌粪便,回肠组织固定并HE染色,16S rRNA技术检测小鼠肠道菌群多样性以及菌群组成。结果:HE染色结果显示,模拟高原低氧组较平原对照组回肠组织损伤明显,血清DAO浓度显著升高(P<0.05),但肠道菌群物种丰度、菌群多样性差异无统计学意义。与模拟高原低氧组相比,高原槲皮素、白藜芦醇组小鼠回肠组织结构得以改善,血清DAO水平降低,且肠道菌群物种丰度、α多样性降低(P<0.05),拟杆菌相对丰度降低(P<0.05),厚壁菌升高(P<0.05),谷氨酸棒状杆菌、罗伊氏乳杆菌相对丰度升高(P<0.05)。结论:槲皮素和白藜芦醇可有效增加小鼠有益菌群的丰度,抑制并降低条件致病菌,对高原低氧暴露小鼠肠道有一定的保护作用。Objective:To investigate the protective effects of three Polyphenolic compounds on intestinal microbial communities in mice exposed intermittent plateau hypoxia.Methods:In this study,60 healthy male Balb/c mice were randomly divided into plain control group,plateau control group,primary anthocyanin intervention group,quercetin intervention group and resveratrol intervention group,12 mice in each group.Primary anthocyanin,quercetin and resveratrol were administrated by gavage at the doses of 50,100 and 20 mg/kg in pharmacological intervention group,respectively.After exposure of the mice to simulation plateau-condition for 30 days,the serum samples were collected for DAO testing,sterile feces were collected in mice,and the diversity and genus level of the mouse gut bacteria were detected by using 16S rRNA technology.Ileum tissue was fixed and stained with HE.Results:HE staining showed that the plateau control group had significant damage to the intestinal tissue structure compared to the plain control group,and the serum DAO concentration was increased(P<0.05),but there was no statistical difference in the abundance and diversity of intestinal flora species.Contrast to simulated intermittent plateau hypoxia group,the structure of the intestine tissue and the level of DAO in the quercetin intervention group and resveratrol intervention group were improved(P<0.05),the abundance andαdiversity of the intestinal flora were decreased,the relative abundance of Bacteroidetes was reduced(P<0.05),and the Firmicutes was increased.Concomitantly,significant decreases in relative abundance were observed for Corynebacterium glutamicum and Lactobacillus reuteri(P<0.05).Conclusion:Quercetin and resveratrol showed some degree of protection to mice intestinal microbial communities,and increased the diversity and the abundance of the dominant flora and inhibited the growth of conditional pathogenic bacteria.

关 键 词：高原低氧 原花青素 槲皮素 白藜芦醇 肠道菌群 

分 类 号：R73-3[医药卫生—肿瘤]