独一味种子生活力最优测定方法研究  

Research on the Optimum Determination Method of the Seed Viability of Lamiophlomis rotata (Benth.) Kudo

作　　者：李廷菊 樊锦雅 王成辉 古锐[2] 钟世红 LI Tingju;FAN Jinya;WANG Chenghui;GU Rui;ZHONG Shihong(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;School of Pharmacy,Southwest Minzu University,Chengdu 610041,China)

机构地区：[1]成都中医药大学药学院,成都611137 [2]成都中医药大学民族医药学院,成都611137 [3]西南民族大学药学院,成都610041

出　　处：《种子》2023年第2期146-149,156,共5页Seed

基　　金：国家科学技术部重点研发计划(2019 YFC 1712305)。

摘　　要：为测定独一味种子生活力及最佳染色条件,建立独一味种子活力测定的快速方法,以野生独一味种子为材料,采用2,3,5-氯化三苯基四氮唑(TTC)染色法,通过单因素试验、正交试验研究不同浸种温度、浸种时间、染色温度、染色时间和TTC浓度对独一味种子生活力测定的影响。结果表明,5个因素对独一味种子生活力的影响依次为:染色时间>染色温度>TTC浓度>浸种时间>浸种温度;正交试验染色最佳条件为25℃水浸泡9 h、0.1%TTC、40℃恒温箱染色9 h。In order to determine the viability and optimal dyeing conditions of Lamiophlomis rotata(Benth.) Kudo seeds,establish a rapid method for the determination of viability of L.rotata(Benth.) Kudo seeds,using wild L.rotata(Benth.) Kudo seeds as experimental materials,using 2,3,5-triphenyltrizole chloride(TTC) dyeing method,through univariate experiments and orthogonal experiments to study the influence of different soaking temperature,soaking time,dyeing temperature,dyeing time and TTC concentration on the determination of L.rotata(Benth.) Kudo seed vitality.The results showed that the effects of the five factors on the viability of the L.rotata(Benth.) Kudo seed from strong to small were the dyeing time,the staining temperature,the TTC concentration,the soaking time,the soaking temperature.The optimal conditions for orthogonal experimental staining were 9 hours immersion in water at 25 ℃,0.1% concentration TTC,and 40 ℃ incubator staining for 9 hours.

关 键 词：独一味 种子 TTC染色法 生活力 

分 类 号：S567.23[农业科学—中草药栽培]