21种药用植物提取物的抑菌活性筛选及其化学成分分析  

Screening on antimicrobial activity of extracts from 21 medicinal plants and analysis of its chemical components

作　　者：梁晶 樊国全[3] 方涛 李进[1] 张军高 周小云[1] 龚静云 雷斌[2] LIANG Jing;FAN Guo-quan;FANG Tao;LI Jin;ZHANG Jun-gao;ZHOU Xiao-yun;GONG Jing-yun;LEI Bin(Xinjiang Crop Chemical Control Engineering Technology Research Center,Institute of Nuclear Technology and Biotechnology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;The Pesticide Trial Production Center,Scientific and Technological Achievements Transformation Center,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;Luntai Fruits Germplasm Resources Garden,Xinjiang Academy of Agricultural Sciencesin,Luntai 841600,China;College of Agricultural,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业科学院核技术生物技术研究所、新疆作物化学调控工程技术研究中心 [2]新疆农业科学院科技成果转化中心农药试制中心,乌鲁木齐830091 [3]新疆农业科学院轮台果树资源圃,轮台841600 [4]新疆农业大学农学院,乌鲁木齐830052

出　　处：《天然产物研究与开发》2023年第4期562-572,共11页Natural Product Research and Development

基　　金：新疆维吾尔自治区重点研发项目(2022B02001-2)。

摘　　要：为了探究新疆植物资源的药用活性,本研究通过菌丝生长速率测定法,以尖孢镰刀菌、立枯丝核菌和拟轮枝镰刀菌等3种棉花致病菌为靶标菌,对21种新疆药用植物提取物的抑菌活性进行筛选与测定,然后采用超高效液相色谱串联质谱(UPLC-MS/MS),以及相关数据库和文献对曼陀罗乙醇提取物质进行分析鉴定。结果表明,21种植物提取物中曼陀罗对3种棉花病原菌均具有较好的抑菌活性效果,并通过液质联用共鉴定曼陀罗乙醇提取物21个化学成分,主要包括10个生物碱类、4个脂质类、2个酚酸类、2个有机酸类和其他类化合物,其中生物碱类化合物为主要成分占总的47.62%,生物碱类物质因具有显著抑菌活性,可能是曼陀罗类物质的主要抑菌活性成分。In order to explore the medicinal activity of plant resources in Xinjiang.In this study,the antifungal activities of extracts from 21 medicinal plants in Xinjiang were screened and determined by mycelium growth rate method,with Fusarium oxysporum,Rhizoctonia solani and Fusarium verticillioides as target fungi.Then,the ethanol extract of Datura stramonium was analyzed and identified by ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS),related databases and literatures.The results showed that among the 21 plant extracts,D.stramonium had good antifungal activity against three cotton pathogens.A total of 21 chemical components of ethanol extract of D.stramonium were identified by LC-MS,The main chemical constituents includedg ten alkaloids,four lipids,two phenolic acids,two organic acids and other compounds.Among them,alkaloids were the main components,accounting for 47.62%of the total.Alkaloids may be the main antifungal components of D.stramonium due to their significant antifungal activity.

关 键 词：新疆药用植物 棉花病害 曼陀罗 抑菌活性 超高效液相色谱串联质谱 

分 类 号：R284[医药卫生—中药学] S432.1[医药卫生—中医学]