蒲公英不同部位可培养内生细菌种类及其与有机酸、黄酮含量的相关性研究  

Study on Species of Culturable Endophytic Bacteria in Different Parts of Taraxacum mongolicum and Their Correlation with Organic Acid and Flavonoid Content

作　　者：李翠羽 王浩森 唐怡 陈曦[1,2] 刘沁柯 欧传州[1,2] 陈志宇 李化瑞 袁侠 黄春萍 Li Cuiyu;WANG Haosen;TANG Yi;CHEN Xi;LIU Xinke;OU Chuanzhou;CHEN Zhiyu;LI Huarui;YUAN Xia;HUANC Chunping(College of Life Sciences,Sichuan Normal University,Chengdu 610066,Sichuan;Plant Functional Genome and Bioinforrnatics Research Center,Sichuan Normal University,Chengdu 610066,Sichuan;Sichuan Gongga Mountain National Nature Resene,Ganzi,626000,Sichuan)

机构地区：[1]四川师范大学生命科学学院,四川成都610101 [2]四川师范大学植物功能基因组及生物信息学研究中心,四川成都610101 [3]四川贡嘎山国家自然保护区,四川甘孜626000

出　　处：《四川师范大学学报（自然科学版）》2023年第3期390-397,共8页Journal of Sichuan Normal University（Natural Science）

基　　金：四川省科技厅重点研发计划(2023YFN0028);国家中医药管理局全国中药资源普查(G2Y-kJS-2018-004)。

摘　　要：为了分离蒲公英的可培养内生细菌,以及探究内生细菌数量分别与蒲公英有机酸和黄酮类物质的相关性,对蒲公英根、茎、叶和花中的内生细菌进行分离、纯化和鉴定.采用高效液相色谱法(HPLC)测定蒲公英不同组织部位有机酸和黄酮类物质的含量,采用SPSS 17.0统计软件分析蒲公英可培养内生细菌数量分别与有机酸和黄酮类物质的相关性.从蒲公英中共分离纯化得到可培养内生细菌22株,根、茎、叶中的内生细菌数量存在差异,其生物活性成分有机酸和黄酮类物质含量存在相关性,其中绿原酸含量与细菌数量呈显著性正相关(P<0.05).In order to isolate the culturable endophytic bacteria from Taraxacum mongolicum and investigate the correlation between the number of endophytic bacteria and the organic acids and flavonoids of Taraxacum mongolicum,the endophytic bacteria in the roots,stems,leaves and flowers of Taraxacum mongolicum were isolated,purified and identified.The contents of organic acids and flavonoids in different tissue parts of Taraxacum mongolicum were determined by high performance liquid chromatography(HPLC),and the correlation between the number of culturable endophytic bacteria and the contents of organic acids and flavonoids was analyzed by SPSS 17.0 statistical software.A total of 22 culturable endophytic bacteria were isolated and purified from Taraxacum mongolicum.The number of endophytic bacteria in roots,stems and leaves were different,and there was a correlation between the contents of bioactive components,organic acids and flavonoids,among which,the content of chlorogenic acid had a significant positive correlation with the number of bacteria(P<0.05).

关 键 词：蒲公英 内生细菌 HPLC 有机酸 黄酮 

分 类 号：Q93[生物学—微生物学] S567.239[农业科学—中草药栽培]