改良脐带血分离方法的质控样管复苏回顾研究  

A Retrospective Study on a Modified Cord Blood Separation Method Using QC Vial Thawing Data

作　　者：吴春燕 陈阳 李星洁[1] 王自波 陈强[1] 赖真阳 WU Chun-yan;CHEN Yang;LI Xing-jie(Sichuan Cord Blood Bank,610000)

机构地区：[1]四川省脐带血造血干细胞库,四川新生命干细胞科技股份有限公司,四川成都610000

出　　处：《临床输血与检验》2023年第2期239-242,共4页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的对比改良脐带血分离方法(改良方法)与传统富浆法(传统方法)对于脐带血造血干细胞质量的影响。方法根据不同体积段改良传统脐带血分离方法,对近年来复苏的脐带血质控样管进行回顾研究,对比方法改良前后一年,两种制备方法获得产品的各项质量参数。结果使用改良方法后,分离后体积由传统方法的(25.68±2.03)mL下降至(22.53±4.24)mL(P<0.05)。红细胞(RBC)去除率由(75.87±11.84)%上升至(82.94±8.68)%(P<0.001)。复苏的质控样管在总有核细胞(TNC)回收、CD34^(+)细胞回收、CD34^(+)细胞活率等方面两组间无统计学差异。集落形成能力检测中,改良方法组的粒单核细胞系集落形成单位(CFU-GM)回收率为(60.00±32.52)%,较传统方法组的(46.97±28.12)%有提高,且差异具有统计学意义(P<0.05)。结论改良脐带血分离方法提高了RBC去除率,减小了冻存体积,复苏脐带血干细胞具有更好的CFU-GM形成能力。Objective To compare the impact on stem cell quality between using modified cord blood separation method(modified method)and using traditional cord blood separation method(traditional method).Methods We developed a modified method,in which different centrifugation parameters were used according to the samples'volume.We collected QC vials'thawing data,which were processed within one year before and after the modification.Test results were compared between these two methods.Results In modified method group,volume after separation decreased from(25.68±2.03)mL to(22.53±4.24)mL(P<0.05).Red blood cells(RBC)removal rate rose from(75.87±11.84)%to(82.94±8.68)%(P<0.001).No statistic difference was observed in total nucleated cells(TNC)recovery,CD34^(+)cell recovery and CD34^(+)cell viability after thawing.In colony formation test,colony forming unit-granulocyte monocyte(CFU-GM)recovery were(60.00±32.52)%and(46.97±28.12)%in modified method group and traditional method group,respectively.The difference was statistically significant(P<0.05).Conclusions The modified cord blood separation method improved RBC removal rate and freezing product volume.After freezing,the stem cell showed higher potency in CFU-GM formation.

关 键 词：脐带血 改良脐带血分离法 质控样管复苏 

分 类 号：R-331[医药卫生]