低氧-复氧对日本囊对虾抗氧化、呼吸代谢及组织结构的影响  

Effects of hypoxia and reoxygenation on antioxidation,respiratory metabolism and tissue structure of Marsupenaeus japonicus

作　　者：赵思哲 花松松 李永闯 江海仪 张庆起 王攀攀 高焕 阎斌伦[1,2,3,4] ZHAO Sizhe;HUA Songsong;LI Yongchuang;JIANG Haiyi;ZHANG Qingqi;WANG Panpan;GAO Huan;YAN Binlun(Jiangsu Key Laboratory of Marine Biotechnology,College of Marine Science and Fisheries,Jiangsu OceanUniversity,Lianyungang Jiangsu222005,China;CoInnovation Center of Jiangsu Marine Bioindustry Technology,Huaihai Institute of Technology,Lianyungang Jiangsu222005,China;Jiangsu Provincial Infrastructure forConservation and Utilization of Agricultural Germplasm,Nanjing210014,China;Jiangsu Institute ofMarine Resources Development,Lianyungang Jiangsu222005,China;Lianyungang Qiming Aquatic Products Co.,Ltd.,Lianyungang Jiangsu222005,China;Jiangsu Haorun Biological IndustryGroup Co.,Ltd.,Taizhou Jiangsu225300,China)

机构地区：[1]江苏海洋大学海洋科学与水产学院,江苏省海洋生物技术重点实验室,江苏连云港222005 [2]江苏省海洋生物产业技术协同创新中心,江苏连云港222005 [3]江苏省农业种质资源保护与利用平台,南京210014 [4]江苏省海洋资源开发研究院,江苏连云港222005 [5]连云港市启明水产有限公司,江苏连云港222100 [6]江苏好润生物产业集团股份有限公司,江苏泰州225300

出　　处：《海洋渔业》2023年第2期150-161,共12页Marine Fisheries

基　　金：苏北科技专项(SZ-LYG202030);连云港市521科研项目(LYG06521202128);江苏省科技项目计划(BE2022360);江苏省优势学科建设工程资助项目(PAPD);江苏省研究生科研与实践创新计划项目(KYCX2021-065)。

摘　　要：为研究低氧-复氧对日本囊对虾(Marsupenaeus japonicus)抗氧化、呼吸代谢能力及组织结构的影响,在胁迫前(0 h)、低氧胁迫(3、6、9、12、24 h)和复氧(36 h)3个阶段的各时间点采集肝胰腺、鳃和肌肉组织,测定了抗氧化酶(CAT、GSH-PX、SOD)、呼吸代谢酶(PK、PFK、SPH、HK)活力及MDA、LD含量,并对0、24、36 h时对虾肝胰腺和鳃组织结构进行了显微观察。结果显示,低氧胁迫阶段各组织中CAT、GSH-PX、SOD酶活力先升高后降低;复氧阶段,上述指标活力均有所上升。各组织中的MDA含量均在低氧胁迫时期呈现下降趋势,并在低氧胁迫24 h时降到最低值,复氧后肝胰腺和鳃中的MDA含量均显著上升(P<0.05)。呼吸代谢方面,低氧胁迫阶段各组织中SDH酶活力显著下降(P<0.05);PFK、PK、HK酶活力肝胰腺和鳃中先上升后下降,而肌肉中3种酶活力显著下降(P<0.05);各组织中的LD含量随着时间的延长而升高。低氧胁迫下肝胰腺和鳃组织结构均发生不同程度的损伤,复氧后肝胰腺结构被进一步破坏,但鳃组织结构损伤程度有所减轻。研究表明,低氧胁迫会对日本囊对虾抗氧化和呼吸代谢能力产生影响,并对肝胰腺、鳃组织结构造成不同程度的损伤。研究结果有助于阐释日本囊对虾时低氧胁迫的响应机制,为日本囊对虾健康养殖提供参考。As one of the necessary factors for the survival of aquatic animals,dissolved oxygen(DO)plays an important role in growth,development and reproduction.Human activities,global warming,water eutrophication and other phenomena have led to the formation of“low oxygen zones”in the ocean,which have seriously damaged the marine ecological environment and endangered the health of aquatic animals.At the same time,in today’s highdensity breeding mode,excessive breeding density and excessive feed feeding are easy to cause transient or persistent hypoxia in the water body.However,relying on the traditional aeration and aeration methods alone can only solve the problem fundamentally and increase the cost of breeding.And with the development of society and the continuous improvement of human living standards,people’s demand for fresh aquatic products is increasing day by day.In the process of transportation,hypoxia is very likely to occur,which will make aquatic animals have a stress response and eventually lead to death,resulting in serious economic losses.Therefore,studying the effects of hypoxic stress on aquaculture species is very necessary to guide the healthy farming and transportation of aquatic animals.Generally speaking,we refer to the water body with dissolved oxygen content below 2 mg·L-1 as hypoxic water body,and in normal water body,the dissolved oxygen content of lower layer water is often the lowest.Marsupenaeus japonicus is an important economically cultured shrimp in China.Compared with Litopenaeus vannamei,the yield per unit area of M.japonicus has always been at a low level.The reason is that its habits of burrowing sand and other benthic lives restrict its highdensity cultivation.The lower dissolved oxygen content in the bottom water will not only affect the survival rate of its breeding,but also affect the growth rate and breeding density to a certain extent.Therefore,in order to explain the effects of hypoxia reoxygenation process on the physiology of M.japonicus,this study took M.japonicu

关 键 词：日本囊对虾 低氧胁迫 复氧 酶活力 组织结构 

分 类 号：S917.4[农业科学—水产科学]