酱香型大曲中产4-乙基愈创木酚芽孢杆菌的筛选、鉴定及特性研究  

Screening, Identification and Characterization of 4-Ethylguaiacol Producing Bacillus Strains from Jiangxiang Daqu

作　　者：王成俊 李玲珊 范梅 刘君 袁思棋 WANG Chengjun;LI Lingshan;FAN Mei;LIU Jun;YUAN Siqi(School of Bioengineering,Sichuan University of Science&Engineering,Yibin,Sichuan 644000;Wuliangye Co.Ltd.,Yibin,Sichuan 644000;Sichuan Provincial Key Laboratory of Liquor-making Biotechnology and Application,Yibin,Sichuan 644000,China)

机构地区：[1]四川轻化工大学生物工程学院,四川宜宾644000 [2]四川宜宾五粮液股份有限公司,四川宜宾644000 [3]酿酒生物技术与应用四川省重点实验室,四川宜宾644000

出　　处：《酿酒科技》2023年第4期45-52,共8页Liquor-Making Science & Technology

基　　金：五粮液集团公司产学研合作项目(CXY2020ZR002);四川省科技厅重点研发项目(No.2021YFS0341);酿酒生物技术及应用四川省重点实验室项目(NJ2017-01);四川理工学院人才引进项目(2017RCL25)。

摘　　要：从酱香型大曲中筛选得到4株产4-乙基愈创木酚(4-EG)的菌株。通过形态学观察、生理生化试验和分子鉴定种属确定产4-EG量较高的一株菌为蜡样芽孢杆菌Bacillus cereus wsp-2-2,产4-EG量达到245.3μg/L。通过耐受性实验研究,结果表明所确定菌株wsp-2-2能够耐受4%vol酒精度、15%的糖度和pH5.5的酸碱度,为后续研究蜡样芽孢杆菌的4-EG生物合成及应用提供了理论基础。Four strains that produce 4-ethylguaiacol(4-EG)were screened out from Jiangxiang Daqu.Morphological observation,physiological biochemical tests and molecular identification were used to identify the strains.The strain with high yield of 4-EG was identified as Bacillus cereus,and its yield of 4-EG reached 245.3μg/L.Tolerance experiments showed that the strain was resistant to 4%vol alcohol,15%sugar and pH 5.5.This study has provided a theoretical basis for subsequent research on the biosynthesis and application of 4-EG in Bacillus cereus.

关 键 词：4-乙基愈创木酚(4-EG) 酱香白酒大曲 蜡样芽孢杆菌 菌株鉴定 耐受性分析 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.1[轻工技术与工程—食品科学与工程]