Exploration of the Potential Mechanism of Gancao Yangyin Decoction on Aging Based on Network Pharmacology  

基于网络药理学探索甘草养阴汤干预衰老的潜在作用机制

作　　者：CHEN Yong CHEN Yanjuan ZHAO Li HE Hui ZHANG Siwei LIU Dongzhou 陈永;陈艳娟;赵莉;何慧;张思为;刘冬舟(深圳市人民医院风湿病和免疫学科/暨南大学第二临床医院,广东深圳518020;暨南大学基础医学院,广东广州510632;深圳市人民医院健康管理中心/暨南大学第二临床医院,广东广州518020;深圳市人民医院中医科/暨南大学第二临床医院,广东广州518020)

机构地区：[1]Department of Rheumatology and Immunology,Shenzhen People's Hospial/the Second Clinical Medical College of Jinan University,Guangzhou,Guangdong 518020,China [2]School of Basic Medicine,Jinan University,Guangzhou,Guangdong 510632,China [3]Health Management Center,Shenzhen People's Hospital/the Second Clinical Medical College of Jinan University,Guangzhou,Guangdong 518020,China [4]Chinese Medicine Department,Shenzhen People's Hospital/the Second Clinical Medical College of Jinan University,Guangzhou,Guangdong 518020,China

出　　处：《Chinese Medicine and Natural Products》2021年第2期1-10,共10页中医学报（英文）

基　　金：We thank for the funding support from the General Project of the National Natural Science Foundation of China(8197060898)。

摘　　要：Objective:To explore the targels and molecular mechanism of Gancao Yangyin Decoction(甘草养阴汤,GCYYD)based on network pharmacology.Methods:The effective chemical components of 7 kinds of Chinese materia medica in GCYYD and their relevant targets were obtai ned through the traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP)and the encyelopedia of traditional Chinese medicine(ETCM).The aging-related targets were obtained through GeneCards database.The targets related to the effective chemical components were mapped with the aging-related targets,and the gene targets of GCY YD for intervening in aging were obtained.The protein interaction network diagram of GCY YD interfering with aging was drawn through String database and Cytoscape 3.7.1 software,and the core target genes were screened.The potential targets obtained were analyzed by gene ontology(GO)biological function enrichment analysis and kyoto encyelopedia of genes and genomes(KEGG)pathway enrichment analysis.Results:Totally 130 effective chemical components of the 7 kinds of Chinese materia medica of GCYYD and 276 related targets were obtained from TCMSP and ETCM databases.Totally 216 aging-related targets were obtained through GeneCards database.There were 63 target genes intervening in aging in GCYYD,with core target genes ALB,AKTI,TNF,L 6,MMP-3,VEGFA,CASP5,etc.Through biological function and signaling pathway enrichment analyses for the target genes with R software,147 KEGG signaling pathways were found,mainly related to age-RAGE signaling pathway in diabetic complications,proteoglycans in cancer,fluid shear stress and atherosclero-sis,HIF-1 signaling pathway,human cytomegalovirus infection,celluar senescence,prostate cancer,bladder cancer,elte.Conclusion:GCYYD can intervene in aging through"multicomponents-mulitargets-multipath-ways",which lays foundation for further experimental research.目的:基于网络药理学初步探究甘草养阴汤干预衰老的作用靶点及分子机制。方法:通过中药系统药理学数据库及分析平台(traditional chinese medicine systems pharmacology database and analysis platform,TCMSP)和中药综合资源数据库(The encyclopedia of traditional chinese medicine,ETCM)获得甘草养阴汤7种中药的有效化学成分及其相关作用靶点。通过GeneCards数据库获取衰老相关靶点。将有效化学成分相关作用靶点与衰老相关靶点进行映射,获取甘草养阴汤干预衰老的基因靶点。采用String数据库及Cytoscape 3.7.1软件绘制甘草养阴汤干预衰老的蛋白互作网络图,并筛选核心靶点基因。将获得的潜在作用靶点进行基因本体(gene ontology,GO)生物功能富集分析和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析。结果:通过TCMSP和ETCM数据库获得甘草养阴汤7种中药有效化学成分130种,相关靶点276个;通过GeneCards数据库获取衰老相关靶点216个;甘草养阴汤干预衰老的靶点基因63个,核心靶点基因包括ALB、AKT1、TNF、IL-6、MMP-3、VEGFA、CASP5等。采用R软件对靶点基因进行生物功能和信号通路富集分析,发现KEGG信号通路147条,主要涉及糖尿病并发症中的年龄-RAGE信号通路(age-RAGE signaling pathway in diabetic complications)、癌症中的蛋白多糖(proteoglycans in cancer)、流体剪切应力和动脉粥样硬化(fluid shear stress and atherosclerosis)、HIF-1信号通路(HIF-1 signaling pathway)、人类巨细胞病毒感染(human cytomegalovirus infection)、细胞衰老(celluar senescence)、前列腺癌(prostate cancer)、膀胱癌(bladder cancer)等。结论:甘草养阴汤可通过“多成分-多靶点-多途径”干预衰老,为进一步实验研究奠定了基础。

关 键 词：Gancao Yangyin Decoction(甘草养阴汤 GCY YD) net work pharmacology AGING action mechanism 

分 类 号：O57[理学—粒子物理与原子核物理]