Microtubes with gradient decellularized porcine sciatic nerve matrix from microfluidics for sciatic nerve regeneration  

作　　者：Binghui Jin Yunru Yu Xiangxiang Chen Yanhong Yang Yushan Xiong Young Jun Im Yuanjin Zhao Jian Xiao 

机构地区：[1]Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine,Vision and Brain Health),School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,Zhejiang Province,325035,China [2]Department of Rheumatology and Immunology,Nanjing Drum Tower Hospital,School of Biological Science and Medical Engineering,Southeast University,Nanjing,210096,China [3]College of Pharmacy,Chonnam National University,Gwangju,61186,Republic of Korea [4]Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou,Zhejiang,325001,China

出　　处：《Bioactive Materials》2023年第3期511-519,共9页生物活性材料（英文）

基　　金：National Key Research and Development Program of China(2020YFA0908200);National Natural Science Foundation of China(81972150,82172428 and 61927805);Natural Science Foundation of Zhejiang(LR18H150001 and LQ22E030004);Shenzhen Fundamental Research Program(JCYJ20190813152616459);Key scientific and technological innovation projects of Wenzhou(ZY20200023);Wenzhou Innovation Team(Growth factor drug development)(No.201801);CAMS Innovation Fund for Medical Sciences(2019-I2M-5-028).

摘　　要：Long-range peripheral nerve defect is a severe and worldwide disease.With the increasing development of tissue engineering,the excellent ability of nerve extracellular matrix(ECM)in peripheral nerve injury(PNI)has been widely studied and verified.Here,we present a novel microtube that contains gradient decellularized porcine sciatic nerve ECM hydrogel(pDScNM-gel)from microfluidics for sciatic nerve regeneration.The pDScNM is confirmed to enhance cell proliferation and migration,and improve the axon growth of primary dorsal root ganglions(DRGs)in a concentration-related manner.These behaviors were also achieved when cells were co-cultured in a gradient pDScNM microtube.The in vivo sciatic nerve regeneration and functional recovery were also demonstrated by assembling the gradient pDScNM microtubes with a medical silicon tube.These results indicated that the microtubes with gradient pDScNM could act as a promising alternative for repairing peripheral nerve defects and showed great potential in clinical use.

关 键 词：MICROFIBER Peripheral nerve injury Extracellular matrix MICROFLUIDICS Nerve regeneration 

分 类 号：R733.7[医药卫生—肿瘤]