Puerarin@Chitosan composite for infected bone repair through mimicking the bio-functions of antimicrobial peptides  

作　　者：Liping Ouyang Baohui Chen Xingdan Liu Donghui Wang Yang Li Yun Liao Kelvin W.K.Yeung Xuanyong Liu 

机构地区：[1]State Key Laboratory of High Performance Ceramics and Superfine Microstructure,Shanghai Institute of Ceramics,Chinese Academy of Sciences,Shanghai,200050,China [2]Hongqiao International Institute of edicine,Shanghai Jiao Tong University School of Medicine,Shanghai,200336,China [3]Cixi Center of Biomaterials Surface Engineering,Ningbo,315300,China [4]Department of Computational Biology for Individualised Medicine,Centre for Individualised Infection Medicine(CiiM)&TWINCORE,Joint Ventures Between the Helmholtz-Centre for Infection Research(HZI)and the Hannover Medical School(MHH),Hannover,Germany [5]Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma,Guangdong Engineering Technology Research Center for Orthopaedic Trauma Repair,Department of Orthopaedics and Traumatology,The University of Hong Kong Shenzhen Hospital,Shenzhen,China

出　　处：《Bioactive Materials》2023年第3期520-530,共11页生物活性材料（英文）

基　　金：National Natural Science Foundation of China(U21A20100,32000938);Science and Technology Commission of Shanghai Municipality,China(19JC1415500,20ZR1465000);Shenzhen Science and Technology Funding(JCYJ20210324120009026);S&T Innovation 2025 Major Special Program of Ningbo(2018B10040)are acknowledged.

摘　　要：It is important to eliminate lipopolysaccharide(LPS)along with killing bacteria in periprosthetic joint infection(PJI)therapy for promoting bone repair due to its effect to regulate macrophages response.Although natural antimicrobial peptides(AMPs)offer a good solution,the unknown toxicity,high cost and exogenetic immune response hamper their applications in clinic.In this work,we fabricated a nanowire-like composite material,named P@C,by combining chitosan and puerarin via solid-phase reaction,which can finely mimic the bio-functions of AMPs.Chitosan,serving as the bacteria membrane puncture agent,and puerarin,serving as the LPS target agent,synergistically destroy the bacterial membrane structure and inhibit its recovery,thus endowing P@C with good antibacterial property.In addition,P@C possesses good osteoimmunomodulation due to its ability of LPS elimination and macrophage differentiation modulation.The in vivo results show that P@C can inhibit the LPS induced bone destruction in the Escherichia coli infected rat.P@C exhibits superior bone regeneration in Escherichia coli infected rat due to the comprehensive functions of its superior antibacterial property,and its ability of LPS elimination and immunomodulation.P@C can well mimic the functions of AMPs,which provides a novel and effective method for treating the PJI in clinic.

关 键 词：Osteoimmunomodulation ANTIBACTERIAL CHITOSAN PUERARIN LIPOPOLYSACCHARIDE 

分 类 号：TB33[一般工业技术—材料科学与工程] R318.08[医药卫生—生物医学工程]