HBV影响甲磺酸阿帕替尼治疗肝细胞癌效果的机制研究  

Mechanism of HBV affecting the therapeutic efficacy of apatinib mesylate on hepatocellular carcinoma

作　　者：赵乐 吴忱思[1] 张瑞星[1] 郭占军[1] ZHAO Yue;WU Chen-si;ZHANG Rui-xing;GUO Zhan-jun(Department of Gastroenterology and Hepatology,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China)

机构地区：[1]河北医科大学第四医院消化内科,河北石家庄050011

出　　处：《河北医科大学学报》2023年第4期382-385,416,共5页Journal of Hebei Medical University

基　　金：河北省重点研发计划(20377726D);河北省医学科学研究重点课题计划(20221221)。

摘　　要：目的 研究乙型肝炎病毒(hepatitis B virus,HBV)感染对阿帕替尼干预下肝细胞癌(hepatocellular carcinoma,HCC)细胞增殖、迁移、凋亡的影响。方法 体外培养阿帕替尼干预的人肝癌HepG2和HepG2.215细胞,细胞计数试剂(cell counting Kit-8,CCK-8)法检测细胞增殖抑制作用,细胞划痕实验检测细胞迁移力,流式细胞术检测细胞凋亡率。结果 阿帕替尼干预后的HepG2细胞组划痕愈合率明显低于HepG2.215细胞,早期凋亡率、晚期细胞凋亡率及总凋亡率均高于HepG2.215细胞组,差异有统计学意义(P<0.05)。结论 HBV状态影响甲磺酸阿帕替尼抑制肝癌细胞迁移和诱导细胞凋亡的效果,阿帕替尼在HBV阴性HCC细胞中可能更好地发挥抗肿瘤作用。Objective To explore the effect of hepatitis B virus(HBV)infection on proliferation,migration and apoptosis of hepatocellular carcinoma(HCC)cells induced by apatinib.Methods Human hepatoma HepG2 and HepG2.215 cells induced by apatinib were cultured in vitro.Cell counting Kit-8(CCK-8)method was used to detect the inhibition of cell proliferation,Cell migration was measured by wound healing assay,and cell apoptosis was detected by flow cytometry.Results After treatment with apatinib,the scratch healing rate of HepG2 cells was significantly lower than that of HepG2.215 cells,while the early apoptosis rate,late apoptosis rate and total apoptosis rate of HepG2 cell group were significantly higher than those of HepG2.215 cell group,showing significant differences(P<0.05).Conclusion HBV affects the efficacy of apatinib mesylate on inhibiting migration and inducing apoptosis of HCC cells.Apatinib may play a better anti-tumor role in non-HBV HCC cells.

关 键 词：癌 肝细胞 甲磺酸阿帕替尼 乙型肝炎病毒 

分 类 号：R735.7[医药卫生—肿瘤]