TP53、NLRP3、miR-551b在胆囊癌组织中的表达及与临床病理特征和预后的关系  

The expression of TP53,NLRP3,and miR-551b in gallbladder cancer tissues and their relationship with clinicopathological characteristics and prognosis

作　　者：周盈 温桂海[2] 史增辉 ZHOU Ying;WEN Gui-hai;SHI Zeng-hui(Department of Hepatobiliary Surgery,Handan Central Hospital,Hebei Province,Handan 056000,China;Department of General Surgery,Handan Central Hospital,Hebei Province,Handan 056000,China;Department of Gastroenterology,Langfang People′s Hospital,Hebei Province,Langfang 065000,China)

机构地区：[1]河北省邯郸市中心医院肝胆外科,河北邯郸056000 [2]河北省邯郸市中心医院普通外科,河北邯郸056000 [3]河北省廊坊市人民医院消化科,河北廊坊065000

出　　处：《河北医科大学学报》2023年第4期423-427,433,共6页Journal of Hebei Medical University

基　　金：河北省医学科学研究课题计划(20200476)。

摘　　要：目的 探究肿瘤抑制蛋白(tumor protein p53,Tp53)、核苷酸结合寡聚化结构域样受体蛋白3(nucleotide-binding oligomerization domain-like receptor protein 3,NLRP3)、微小核糖核酸551b(miR-551b)在胆囊癌组织中的表达及与临床病理特征和预后的关系。方法 选取在我院进行胆囊切除术的50例胆囊癌患者切除的胆囊癌组织为胆囊癌组,以及50例癌旁组织标本为对照组,同时期在我院接受胆囊炎治疗的50例为胆囊炎组。采用荧光定量聚合酶链反应测定miR-551b、TP53、NLRP3表达。观察P53、NLRP3、miR-551b表达在各组之间的表达,分析三者与临床病理和预后的关系,三者的相关性。结果 胆囊癌组TP53、NLRP3均高于对照组、胆囊炎组,miR-551b低于对照组(P<0.05)。对照组TP53、NLRP3低于胆囊炎组,miR-551b高于胆囊炎组(P<0.05)。随临床分期程度加重、分化程度越低、直径越大TP53、NLRP3表达不断升高,miR-551b表达不断降低(P<0.05)。有淋巴结转移患者TP53、NLRP3表达高于无淋巴结转移,miR-551b低于无淋巴结转移(P<0.05)。预后不良TP53、NLRP3表达高于预后良好,miR-551b低于预后良好(P<0.05)。TP53与NLRP3呈正相关,miR-551b与NLRP3、TP53呈负相关(P<0.05)。结论 P53、NLRP3在胆囊癌组织中高表达,miR-551b低表达,三者与临床特征、预后具有一定的相关性,三者之间具有相关性,高表达TP53、NLRP3以及低表达miR-551b是胆囊癌发病的风险预测因子。Objective To explore the expression of tumor protein p53(Tp53),nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3),and microRNA-551b(miR-551b)and its relationship with clinicopathological characteristics and prognosis in gallbladder cancer tissues.Methods In this study,gallbladder cancer tissues from 50 gallbladder cancer patients who underwent cholecystectomy were selected as the gallbladder cancer group,and 50 adjacent tissues were selected as the control group.Meanwhile,50 patients with cholecystitis treated in our hospital during the same period were selected as the cholecystitis group.The expression of miR-551b,TP53 and NLRP3 was determined by fluorescence quantitative PCR.The expression of P53,NLRP3 and miR-551b between the groups was observed,and the relationship of the three indicators with clinicopathology and prognosis,as well as the correlation of the three was analyzed.Results TP53 and NLRP3 were all higher in the gallbladder cancer group than in the control group and cholecystitis group,while miR-551b was lower than that in the control group(P<0.05).TP53 and NLRP3 were lower in the control group than in the cholecystitis group,while miR-551b was higher than that in the cholecystitis group(P<0.05).The aggravation of clinical stage,lower differentiation and larger diameter led to continuously higher TP53 and NLRP3 expression,and continuously decreased miR-551b expression(P<0.05).The expression of TP53 and NLRP3 in patients with lymph node metastasis was higher than that without lymph node metastasis,and miR-551b was lower than that without lymph node metastasis(P<0.05).The expression of TP53 and NLRP3 in patients with poor prognosis was higher than that of patients with good prognosis,and miR-551b was lower than that of patients with good prognosis(P<0.05).TP53 was positively correlated with NLRP3,and miR-551b was negatively correlated with NLRP3 and TP53(P<0.05).Conclusion TP53 and NLRP3 are highly expressed,while miR-551b is lowly expressed in gallbladder cancer tissues.The three

关 键 词：胆囊肿瘤 肿瘤抑制蛋白类 预后 

分 类 号：R735.8[医药卫生—肿瘤]