大气压低温等离子体对采后桑椹大肠埃希菌的杀灭作用  

Killing Effect of Cold Atmospheric Plasma on Escherichia coli from Postharvest Mulberry Fruit

作　　者：黄孟璐 赵璨 陈佩瑶 刘寅欣 孙翠 华舒浩 黄凌霞[1,3] Huang Menglu;Zhao Can;Chen Peiyao;Liu Yinxin;SSun Cui;Hua Shuhao;Huang Lingxia(College of Animal Sciences,Zhejiang University,Hangzhou 310058,China;Institute of Fruit Science,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China;Zhejiang Key Laboratory of Silkworm and Bee Resources Utilization and Innovation Research,Hangzhou 310058,China)

机构地区：[1]浙江大学动物科学学院,杭州310058 [2]浙江大学农业与生物技术学院果树科学研究所,杭州310058 [3]浙江省蚕蜂资源利用与创新研究重点实验室,杭州310058

出　　处：《蚕业科学》2023年第1期82-90,共9页ACTA SERICOLOGICA SINICA

基　　金：浙江省基础公益研究计划项目(LGN21C200016);国家重点研发计划项目(2017YFE0122300);浙江省农业重大技术协同推广计划项目(2022XTTGCS03)。

摘　　要：食品表面的大肠埃希菌(Escherichia coli)对消费者的健康构成威胁,而大气压低温等离子体技术(cold atmospheric plasma,CAP)作为一种新兴的非热杀菌技术,已在食品杀菌保鲜领域得到广泛应用。为探究CAP对E.coli的杀灭作用及机制,文中以采后桑椹表面E.coli为试验菌株,研究了CAP对E.coli的抑制效果及对其细胞膜、亚显微结构、胞内活性氧(reactive oxygen species,ROS)积累和细胞凋亡情况的影响,探讨了CAP技术对E.coli的杀菌机制。结果表明,CAP处理可显著抑制E.coli的生长,造成其细胞膜破损及部分细胞裂解,同时引起E.coli细胞内ROS的积累,进一步诱导其发生细胞凋亡,E.coli细胞受损程度、ROS积累趋势和凋亡率均随电流强度增加而增加。由此推测,CAP可能通过对E.coli细胞膜的损伤引起胞内生物学损伤,破坏细胞活性,从而对其起到抑制和杀灭效果。Escherichia coli on the surface of food poses a threat to the health of consumers.As an emerging non-thermal sterilization technology,CAP(cold atmospheric plasma)technology has been widely used in the field of food sterilization and preservation.In order to explore the kiling effect and mechanism of CAP on E.coli,the E.coli on the surface of postharvest mulberry fruit was used as the test strain to study the inhibitory effect of CAP on E.coli and its effect on the cell membrane,submicroscopic structure,ROs(reactive oxygen species)accumulation and cell apoptosis.The bactericidal mechanism of CAP technology on E.coli was also discussed.The results showed that CAP treatment could significantly inhibit the growth of E.coli,causing damage to its cell membrane and partial cell lysis,and at the same time caused the accumulation of ROS in E.coli cells,which further induced the cell apoptosis.The damage of E.coli cells,the ROS accumulation trend and the apoptosis rate all increased with the increase of current intensity.It is speculated that CAP may inhibit and kill E.coli by damaging its cell membrane to cause intracellular biological damage and reduce its cell activity.

关 键 词：大气压低温等离子体 大肠埃希菌 杀菌 细胞膜 胞内活性氧 

分 类 号：TS205[轻工技术与工程—食品科学]