JMJD3与CyclinD1基因在喉鳞状细胞癌组织中的表达及意义  

Expressions and significance of JMJD3 and CyclinD1 in cancer tissues of laryngeal squamous cell carcinoma

作　　者：刘玉娟 郭明艳 LIU Yujuan;GUO Mingyan(Department of Otolaryngology,Yangling Demonstration District Hospital,Xianyang,Shaanxi 712100,China;Department of Clinical Laboratory,Yangling Demonstration District Hospital,Xianyang,Shaanxi 712100,China)

机构地区：[1]杨凌示范区医院耳鼻咽喉科,陕西咸阳712100 [2]杨凌示范区医院检验科,陕西咸阳712100

出　　处：《检验医学与临床》2023年第8期1126-1129,共4页Laboratory Medicine and Clinic

摘　　要：目的探讨Jumonji结构域包含蛋白3(JMJD3)与细胞周期素D1(CyclinD1)基因在喉鳞状细胞癌组织中的表达及意义。方法选取2016年12月至2021年12月该院肿瘤科收治的80例喉鳞状细胞癌患者作为研究对象,术中取喉鳞状细胞癌组织与癌旁正常组织,采用免疫组织化学染色法检测JMJD3和CyclinD1基因的表达情况,分析JMJD3和CyclinD1表达与临床病理特征的关系。结果喉鳞状细胞癌组织与癌旁正常组织JMJD3阳性率分别为48.75%、17.50%,CyclinD1阳性率分别为60.00%、20.00%,喉鳞状细胞癌组织JMJD3和CyclinD1的表达均高于癌旁正常组织,差异均有统计学意义(P<0.05);不同年龄、性别、吸烟史的喉鳞状细胞癌患者JMJD3和CyclinD1阳性率比较,差异均无统计学意义(P>0.05);非声门型、分化程度为中低分化、有淋巴结转移及TNM分期为Ⅲ~Ⅳ期的喉鳞状细胞癌患者JMJD3与CyclinD1阳性率更高;癌组织JMJD3与CyclinD1表达呈正相关(r=0.661,P<0.05)。结论JMJD3和CyclinD1基因在喉鳞状细胞癌组织中均呈过度表达,并且二者呈正相关,说明与癌细胞的病情进展、侵袭性、恶性程度有关,在癌细胞的发展过程中起重要作用。Objective To investigate the expressions and significance of Jumonji domain-containing protein 3(JMJD3)and CyclinD1 in tissues of laryngeal squamous cell carcinoma.Methods A total of 80 patients with laryngeal squamous cell carcinoma treated in department of oncology of the hospital from December 2016 to December 2021 were selected.Laryngeal cancer tissues and adjacent tissues were collected during surgery,and immunohistochemical staining was used to detect the protein expressions of JMJD3 and CyclinD1.The relationship between the expressions of JMJD3 and CyclinD1 and the clinicopathological characteristics were analyzed.Results The positive rates of JMJD3 in laryngeal squamous cell carcinoma tissues and adjacent normal tissues were 48.75%and 17.50%,and the positive rates of CyclinD1 were 60.00%and 20.00%respectively.The expressions of JMJD3 and CyclinD1 in laryngeal squamous cell carcinoma tissues were higher than those in adjacent normal tissues(P<0.05).There were no statistical differences in age,gender and smoking history in patients with laryngeal squamous cell carcinoma(P>0.05).The positive rates of JMJD3 and CyclinD1 were higher among laryngeal squamous cell carcinoma patients with glottic type,moderate or poor differentiation degree,lymph node metastasis andⅢ-ⅣTNM stage(P<0.05).The expression of JMJD3 in cancer tissues was positively correlated with CyclinD1 expression(r=0.661,P<0.05).Conclusion Both JMJD3 and CyclinD1 genes are over-expressed in laryngeal squamous cell carcinoma tissues,and they are positively correlated with each other,indicating that they are related to the progression,invasiveness and malignancy of cancer cells,and play an important role in the development of cancer cells.

关 键 词：喉鳞状细胞癌 Jumonji结构域包含蛋白3 细胞周期素D1 

分 类 号：R739.65[医药卫生—肿瘤]