Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure  

作　　者：Zhe Liu Qian Tang Ruo-Tao Liu Ming-Zhao Yu Hao Peng Chang-Qing Zhang Zhen-Zhong Zhu Xiao-Juan Wei 

机构地区：[1]Department of Orthopedic Surgery,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,600 Yishan Road,Shanghai,200233,China [2]Institute of Microsurgery on Extremities,Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,600 Yishan Road,Shanghai,200233,China

出　　处：《Bioactive Materials》2023年第4期60-73,共14页生物活性材料（英文）

基　　金：This work was supported by Shanghai Sailing Program(22YF1433200);the National Natural Science Foundation of China(82172400).

摘　　要：Implant failure,which is commonly associated with failure of osseointegration and peri-implant infection,is a severe complication of orthopedic surgery.In particular,the survival rate of implants is significantly decreased in patients using long-term glucocorticoids(GCs).However,the exact molecular mechanism underlying GCs-induced implant loosening,as well as preventive strategies for these patients,is unclear.To address this problem,we performed RNA-sequencing and found that WNT16 was correlated with GCs-induced osteopenia(LogFC=-5.17,p<0.01).Inspired by the concept of“organic-inorganic”hybrid,we theorized to introduce a bioactive two-dimensional nanosheet into a layer-by-layer(LbL)self-assembly coating to construct a customized implant that targets WNT16.After screening commercially available nanosheets,laponite(LAP)was identified as a cost-effective rescuer for GCs-induced WNT16 inhibition,which was then intercalated into LbL deposition system consisting of quaternized chitosan(QCS)and hyaluronic acid(HA).The hybrid coating(QCS/HA/LAP)showed micrometer thickness and improved hydrophilicity and interface roughness.Furthermore,QCS/HA/LAP coated polyetheretherketone(PEEK)implant enhanced cell viability,adhesion,and osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs),and promoted osteointegration of PEEK in GCs-treated rats by targeting the WNT16/β-catenin axis.The assembled QCS has proven antibacterial properties,and the hybrid coating exerted potent detrimental effects against methicillin-resistant Staphylococcus aureus(MRSA)and Escherichia coli(E.coli),both in vitro and in vivo.Taken together,these results suggest that QCS/HA/LAP coating has great potential for use in implants customization,and has synergistic pro-osteogenic and antibacterial effects that help prevent implant failure in GCs-treated patients.

关 键 词：Layer-by-layer self-assembly Organic-inorganic hybrid GLUCOCORTICOID OSSEOINTEGRATION Antibacterial 

分 类 号：TG17[金属学及工艺—金属表面处理]