Upconverting nanoparticle-containing erythrocyte-sized hemoglobin microgels that generate heat,oxygen and reactive oxygen species for suppressing hypoxic tumors  

作　　者：Hanju Kim Johyun Yoon Hwang Kyung Kim Woo Tak Lee Nguyen Thi Nguyen Xuan Thien Le Eun-Hee Lee Eun Seong Lee Kyung Taek Oh Han-Gon Choi Yu Seok Youn 

机构地区：[1]School of Pharmacy,Sungkyunkwan University,2066 Seobu-ro,Jangan-gu,Suwon,Gyeonggi-do,16419,Republic of Korea [2]College of Pharmacy,Korea University,2511 Sejong-ro,Sejong,30019,Republic of Korea [3]Department of Biotechnology and Department of Biomedical-Chemical Engineering,the Catholic University of Korea,43 Jibong-ro,Bucheon-si,Gyeonggi-do,14662,Republic of Korea [4]College of Pharmacy,Chung-Ang University,84 Heukseok-ro,Dongjak-gu,Seoul,06974,Republic of Korea [5]College of Pharmacy,Hanyang University,55,Hanyangdaehak-ro,Sangnok-gu,Ansan,15588,Republic of Korea

出　　处：《Bioactive Materials》2023年第4期112-126,共15页生物活性材料（英文）

基　　金：This work was supported by a National Research Foundation of Korea(NRF)grants funded by the Korean government(No.NRF-2019R1A5A2027340);the Bio&Medical Technology Development Program of the National Research Foundation(NRF)funded by the Korean government(MSIT)(No.NRF-2022M3A9G8017220).

摘　　要：Inspired by erythrocytes that contain oxygen-carrying hemoglobin(Hb)and that exhibit photo-driven activity,we introduce homogenous-sized erythrocyte-like Hb microgel(μGel)systems(5-6μm)that can(i)emit heat,(ii)supply oxygen,and(iii)generate reactive oxygen species(ROS;1O2)in response to near-infrared(NIR)laser irradiation.Hb μGels consist of Hb,bovine serum albumin(BSA),chlorin e6(Ce6)and erbium@lutetium upconverting nanoparticles(UCNPs;~35 nm)that effectively convert 808 nm NIR light to 660 nm visible light.These Hb μGels are capable of releasing oxygen to help generate sufficient reactive oxygen species(^(1)O_(2))from UCNPs/Ce6 under severely hypoxic condition upon NIR stimulation for efficient photodynamic activity.Moreover,the Hb μGels emit heat and increase surface temperature due to NIR light absorption by heme(iron protoporphyrin IX)and display photothermal activity.By changing the Hb/UCNP/Ce6 ratio and controlling the amount of NIR laser irradiation,it is possible to formulate bespoke Hb μGels with either photothermal or photodynamic activity or both in the context of combined therapeutic effect.These Hb μGels effectively suppress highly hypoxic 4T1 cell spheroid growth and xenograft mice tumors in vivo.

关 键 词：Hemoglobin microgel Upconversion Photothermal therapy Photodynamic therapy Hypoxic tumors Oxygen-generation 

分 类 号：R73[医药卫生—肿瘤]