A-PRF联合Bio-Oss对种植体周围骨缺损的修复效果实验研究  

Experimental Study on the Effect of A-PRF Combined with Bio-Oss in Repairing Peri-implant Bone Defects

作　　者：黄仙波 何志良[1] 曹雷[1] 贺成功[1] 马慧颖 李燕波[1] 李士辉 李春佳[1] 张艳波[2] 田茜 HAUNG Xianbo;HE Zhiliang;CAO Lei;HE Chenggong;MA Huiying;LI Yanbo;LI Shihui;LI Chunjia;ZHANG Yanbo;TIAN Qian(Department of Stomatology,Chengde Central Hospital,Chengde,Hebei 067000,China;Department of Stomatology,the Affiliated Hospital of Chengde Medical University,Chengde,Hebei 067000,China;Department of Stomatology,the Hospital of Wen'an County,Wen'an,Hebei 065800,China)

机构地区：[1]承德市中心医院口腔科,河北承德067000 [2]承德医学院附属医院口腔科,河北承德067000 [3]文安县医院口腔科,河北文安065800

出　　处：《临床误诊误治》2023年第4期136-141,共6页Clinical Misdiagnosis & Mistherapy

基　　金：河北省医学科学研究课题计划(20211447);承德市科学技术研究与发展计划项目(202204A147)。

摘　　要：目的探讨改良型富血小板纤维蛋白(A-PRF)联合Bio-Oss对种植体周围骨缺损的修复效果。方法12只新西兰大白兔建立下颌骨缺损模型,在每只大白兔双侧下颌骨制备6个骨缺损孔,并分别植入A-PRF与Bio-Oss骨粉2∶1混合物(A组)、1∶1混合物(B组)、1∶2混合物(C组)、空白对照(D组)、单独植入A-PRF(E组)、单独植入Bio-Oss骨粉(F组),于植入后4、8、12周处死动物,行大体观察、影像学及组织学观察,并对成骨效果进行分析。结果术后4周,D组可见纤维结缔组织围绕,A组、E组骨质完全覆盖螺纹钉,其余3组出现不同程度骨质覆盖,术后8、12周相同部位种植体表面骨质覆盖增厚。术后4、8、12周骨体积分数A组>E组>B组>C组>F组>D组(P<0.05)。术后4周,A组有新生血管、骨基质形成,骨小梁钙化良好,有大量新骨形成;术后8、12周,A组骨小梁融合成岛状,可见不同程度的板状骨。术后4、8、12周骨形成率A组>E组>B组>C组>F组>D组(P<0.05)。结论A-PRF与Bio-Oss混合物具有良好骨缺损修复效果,A-PRF与Bio-Oss 2∶1混合物成骨效果更佳,可提高种植体骨愈合能力。Objective To explore the experimental study on the effect of modified platelet-rich fibrin(A-PRF)combined with Bio-Oss in repairing peri-implant bone defects.Methods A mandibular defect model was established in 12 New Zealand white rabbits.Six bone defect holes were prepared in the bilateral mandibles of each rabbit and implanted with a 2∶1 mixture of A-PRF and Bio-Oss bone powder(Group A),a 1∶1 mixture(Group B),a 1∶2 mixture(Group C),a blank control(Group D),a single implantation of A-PRF(Group E),and a single implantation of Bio-Oss bone powder(Group F),respectively.The animals were sacrificed at 4,8,12 weeks after implantation for gross observation,imaging and histological observation,and analysis of osteogenic effects.Results At 4 weeks after surgery,fibrous connective tissue could be seen surrounding the implant in Group D,and bone in Group A and Group E completely covered the screw.Bone coverage in the other 3 groups appeared in varying degrees,and at 8 and 12 weeks after surgery,the bone coverage on the surface of the implant at the same location was thicker.At 4,8,and 12 weeks after surgery,the bone volume fraction was the greatest in Group A,followed by Group E,Group B,Group C,Group F,and Group D(P<0.05).At 4 weeks after surgery,in Group A,there were neovascularization,bone matrix formation,good bone trabecular calcification,and a large amount of new bone formation.At 8 and 12 weeks after surgery,the trabeculae in Group A were fused to form islands,with different degrees of plate-like bone visible.At 4,8,12 weeks after surgery,the bone formation rate was the greatest in Group A,followed by Group E,Group B,Group C,Group F and Group D(P<0.05).Conclusion The mixture of A-PRF and Bio-Oss has a good effect on bone defect repair,especially a 2∶1 mixture of A-PRF and Bio-Oss bone,showing better osteogenic effects,which can improve the healing ability of implant bone.

关 键 词：改良型富血小板纤维蛋白 BIO-OSS 下颌骨 骨缺损 牙种植体 修复 骨体积分数 骨生成 

分 类 号：R-332[医药卫生] R782