大鼠内侧隔核谷氨酸能神经元的纤维投射  

Projections of glutamatergic neurons in medial septum in the rat

作　　者：齐争艳 孙佳 马琳[1,2] 马文倩 赵梦 王正华 李昌硕 文玉军 QI Zhengyan;SUN Jia;MA Lin;MA Wenqian;ZHAO Meng;WANG Zhenghua;LI Changshuo;WEN Yujun(Department of Human Anatomy,Histology and Embryology,School of Basic Medicine,Ningxia Medical University,Yinchuan 750004,China;Key Laboratory of Craniocerebral Diseases,Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学基础医学院人体解剖与组织胚胎学系,银川750004 [2]宁夏医科大学颅脑疾病重点实验室,银川750004

出　　处：《神经解剖学杂志》2023年第1期47-53,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(32160191);宁夏重点研发计划项目(2021BEG03096)。

摘　　要：目的:通过立体定位注射重组腺相关病毒(rAAV)方法观察大鼠内侧隔核(MS)中谷氨酸能神经元的纤维投射。方法:将rAAV2/9-CaMKⅡa-EGFP、rAAV/retro-hSyn-mCherry病毒混合后注射到大鼠MS,待病毒在大鼠体内表达3周后灌注取脑、切片观察,检测MS的纤维投射情况。结果:rAAV2/9-CaMKⅡa-EGFP病毒主要标记到齿状回、黑质、红核、丘脑、乳头体上核,海马的CA2、CA3区;rAAV/retro-hSyn-mCherry病毒主要标记到背侧海马和内梨形背侧核、伏核神经元,少部分标记到第一躯体感觉皮层、第二视皮质和海马的CA1区神经元。结论:大鼠MS谷氨酸能神经元投射的脑区主要在边缘系统、基底节区以及海马,海马和部分皮层有纤维投射到MS。Objective:Recombinant adeno-associated virus(rAAV)were injected into the medial septum(MS)of rats by stereotactic injection,and the projections of glutamatergic neurons in MS were investigated.Methods:The mixed rAAV2/9-CaMKIIa-EGFP and rAAV/retro-hSyn-mCherry were injected into MS of rats.After the virus were expressed in rats for 3 weeks,the rat brain was taken and sectionalized for observation to detect the projections in MS.Results:rAAV2/9-CaMKⅡa-EGFP virus were mainly labeled in the dentate gyrus,substantia nigra,red nucleus,thalamus,supramammillary nucleus,and the CA2 and CA3 regions of the hippocampus;rAAV/retro-hSyn-mCherry virus were primarily labeled in the dorsal hippocampus,dorsal endopiriform nucleus and accumbens nucleus.A few of them were marked to the primary somatosensory cortex,secondary visual cortex,and the CA1 of the hippocampus.Conclusion:Glutamatergic neurons of MS project mainly to the limbic system,the basal ganglia and the hippocampus,and the hippocampus and parts of cortex project to the MS in rat.

关 键 词：内侧隔核 重组腺相关病毒 谷氨酸能神经元 神经示踪 大鼠 

分 类 号：R338[医药卫生—人体生理学]