不同年限沼灌稻田土壤汞甲基化微生物群落特征及影响因素——以重庆市长寿区某养殖场周边沼灌稻田为例  

Characteristics and Influencing Factors of Mercury Methylated Microbial Communities in Rice Paddy Soil with Biogas Slurry Irrigation in Different Years--A Case Study of Bog Irrigated Paddy Field Around a Breeding Farm in Changshou District,Chongqing

作　　者：高润霞 刘宇 罗强 张慧[2] 胡廷英 GAO Run-xia;LIU Yu;LUO Qiang;ZHANG Hui;HU Ting-ying(Observation&Research Station of Ecological Restoration for Chongqing Typical Mining Areas,Ministry of Natural Resources(Chongqing Institute cf Geology&Mineral Resources),Chongqing 401120,China;Chongqing Academy of Agricultural Sciences,Chongqing 401329,China)

机构地区：[1]自然资源部重庆典型矿区生态修复野外科学观测研究站(重庆地质矿产研究院),重庆401120 [2]重庆市农业科学院,重庆401329

出　　处：《四川环境》2023年第2期30-35,共6页Sichuan Environment

基　　金：重庆市科委基础与前沿项目(CSTC2018JCYJAX0303)。

摘　　要：稻米中的甲基汞主要来源于稻田土壤,而土壤中无机汞的甲基化与汞甲基化微生物密切相关。对重庆市长寿区某养殖场周边不同年限沼灌稻田土壤样本进行Illumina高通量测序,探讨了不同年限沼灌稻田土壤汞甲基化微生物群落特征,并分析了其与影响因子之间的关系。结果表明,随着沼灌年限的增加,稻田土壤中甲基汞含量有逐渐增加的趋势,与汞甲基化相关微生物如硫酸盐还原菌、铁还原菌以及产甲烷菌在门、纲、目、科、属水平上的占比增大。土壤pH(F=0.208,P=0.00)对不同年限沼灌稻田土壤汞甲基化相关微生物占比的影响最大,其次是土壤有机质(F=0.173,P=0.00)。建议通过提高稻田土壤的pH和降低沼液中含硫有机物的含量等措施减少土壤中无机汞的甲基化。本研究丰富了对沼灌稻田土壤汞甲基化相关微生物的认识,为沼液稻田利用可能带来的汞环境风险的防控提供了理论依据。Methylmercury in rice mainly comes from paddy soil,and the methylation of inorganic mercury in soil is closely relatedto mercury methylated microorganisms.In this study,Illuinina high-throughput sequencing was performed on soil samples from different years of biogas-irrigation paddy field around a farm in Changshou District of Chongqing.The characteristics of soil mercury methylated microbial communities in different years of biogas-irrigation paddy field were discussed,and the relationship between mercury methylated microbial community and influencing factors was analyzed.The results showed that with the increase of biogas irrigation years,the content of methylmercury in paddy soil increased gradually,and the proportion of mercury methylation-related microorganisms such as sulfate-reducing bacteria,iron-reducing bacteria and methanogens at the phylum,class,order,family and genus levels increased.Soil pH(F=0.208,P=0.00)had the greatest impact on the proportion of soil mercury methylation-related microorganisms in different years of biogas irrigation paddy field,followed by soil organic matter(F=0.173,P=0.00).It is suggested to reduce the methylation of inorganic mercury in soil by increasing the pH of paddy soil and reducing the content of sulfur-containing organic matter in biogas slurry.This study enriched the understanding of soil mercury methylation related microorganisms in biogas slurry paddy fields,and provided a theoretical basis for the prevention and control of mercury environmental risks caused by the utilization of biogas slurry paddy fields.

关 键 词：沼液 稻田 汞 甲基化 微生物 

分 类 号：X171[环境科学与工程—环境科学]