IFIT3对鼻咽癌CNE-2R细胞侵袭转移及EMT的影响  

Effects of IFIT3 on invasion,metastasis and EMT of nasopharyngeal carcinoma CNE⁃2R cells

作　　者：覃月兰 孙永楚 陈凯华[1] 宋阳光 刘滢滢 朱小东[1,2] QIN Yuelan;SUN Yongchu;CHEN Kaihua;SONG Yangguang;LIU Yingying;ZHU Xiaodong(Department of Radiation Oncology,Guangxi Medical University Cancer Hosptial,Nanning 530021,China;Department of Oncology,Wuming Hospital of Guangxi Medical University,Nanning 530100,China)

机构地区：[1]广西医科大学附属肿瘤医院放疗科,南宁530021 [2]广西医科大学附属武鸣医院肿瘤科,南宁530100

出　　处：《中国癌症防治杂志》2023年第2期143-148,共6页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：国家自然科学基金项目(81760544);广西区域性高发肿瘤早期防治研究重点实验室项目(GKE-ZZ202230)。

摘　　要：目的探讨干扰素诱导的具有四肽重复序列3(interferon induced protein with tetratricopeptide repeats 3,IFIT3)在鼻咽癌CNE-2R细胞中的表达及其对细胞侵袭、转移和上皮-间质转化(epithelial-mesenchymal transition,EMT)能力的影响。方法采用RT-qPCR和Western blot检测IFIT3在鼻咽癌细胞CNE-2R和正常鼻咽上皮细胞NP69中的表达水平。鼻咽癌CNE-2R细胞分别感染IFIT3 shRNA的3个序列LV1、LV2、LV3,并设立阴性对照组(NC组);利用RT-qPCR和Western blot检测上皮表型E-cadherin及间质表型N-cadherin和Vimentin的表达,采用划痕实验及Transwell实验检测细胞的迁移和侵袭能力。结果IFIT3在鼻咽癌CNE-2R细胞中的mRNA和蛋白表达水平均较NP69细胞显著上调(均P<0.001)。IFIT3在LV1组、LV2组和LV3组中的mRNA和蛋白表达水平均低于NC组(均P<0.001),且以LV2组和LV3组的表达水平最低。与NC组相比,LV2组和LV3组的细胞迁移率显著下降,迁移和侵袭细胞数显著减少,E-cadherin蛋白表达水平显著升高,N-cadherin和Vimentin蛋白表达水平显著降低(均P<0.001)。结论IFIT3在鼻咽癌CNE-2R细胞中表达上调,沉默IFIT3后CNE-2R细胞的侵袭、转移能力以及EMT显著被抑制。Objective To investigate the expression of interferon induced protein with tetratricopeptide repeats 3(IFIT3)in nasopharyngeal carcinoma CNE‑2R cells and its effect on the ability of invasion,metastasis and epithelial‑mesenchymal transition(EMT).Methods The expression levels of IFIT3 in nasopharyngeal carcinoma CNE‑2R cells and normal nasopharyngeal NP69 cells were detected by RT‑qPCR and Western blot.The nasopharyngeal carcinoma cells CNE‑2R were infected with IFIT3 shRNA sequences LV1,LV2 and LV3,and the negative control group(NC group)was set up.The expression of epithelial marker E‑cadherin,mesenchymal markers N‑cadherin and Vimentin were detected by RT‑qPCR and Western blot.The migration and invasion ability of cells were detected by the scratch assay and the Transwell assay.Results ComparedwithNP69cells,themRNA andproteinexpressionlevelsofIFIT3weresignificantlyup‑regulated in CNE‑2R cells(all P<0.001).The mRNA and protein expression levels of IFIT3 in the LV1,LV2 and the LV3 groups were the lower than those in the NC group(all P<0.001)and the expression levels were the lowest in the LV2 and LV3 groups.Compared with the NC group,the cell migration rate,the number of migrating and invasive cells,the expression ofN‑cadherin and Vimentin protein were significantly decreased in both the LV2 and LV3 groups(all P<0.001),while the expression of E‑cadherin protein was significantly increased(all P<0.001).Conclusions sThe expression of IFIT3 is up‑regulated in nasopharyngeal carcinoma CNE‑2R cells,and the invasion and metastasis ability of CNE‑2R cells and EMT are significantly inhibited after IFIT3 silencing.

关 键 词：鼻咽癌 IFIT3 CNE-2R细胞 侵袭 转移 上皮-间质转化 

分 类 号：R739.63[医药卫生—肿瘤]