Biomimetic AgNPs@antimicrobial peptide/silk fibroin coating for infection-trigger antibacterial capability and enhanced osseointegration  

作　　者：Wenhao Zhou Tian Bai Lan Wang Yan Cheng Dandan Xia Sen Yu Yufeng Zheng 

机构地区：[1]Shaanxi Key Laboratory of Biomedical Metallic Materials,Northwest Institute for Non-ferrous Metal Research,Xi’an,710016,China [2]Department of Dental Materials,Peking University School and Hospital of Stomatology,Beijing,100081,China [3]School of Materials Science and Engineering,Peking University,Beijing,100871,China

出　　处：《Bioactive Materials》2023年第2期64-80,共17页生物活性材料（英文）

基　　金：supported by the National Natural Science Foundation of China(Grant numbers 32071327,32101087);Shaanxi Science and Technology Association(2022JQ-312).

摘　　要：Endowing implant surfaces with combined antibacterial and osteogenic properties by drug-loaded coatings has made great strides,but how to achieve the combined excellence of infection-triggered bactericidal and in vivo-proven osteogenic activities without causing bacterial resistance still remains a formidable challenge.Herein,antimicrobial peptides(AMPs)with osteogenic fragments were designed and complexed on the surface of silver nanoparticle(AgNP)through hydrogen bonding,and the collagen structure-bionic silk fibroin(SF)was applied to carry AgNPs@AMPs to achieve infection-triggered antibacterial and osteointegration.As verified by TEM,AMPs contributed to the dispersion and size-regulation of AgNPs,with a particle size of about 20 nm,and a clear protein corona structure was observed on the particle surface.The release curve of silver ion displayed that the SF-based coating owned sensitive pH-responsive properties.In the antibacterial test against S.aureus for up to 21 days,the antibacterial rate had always remained above 99%.Meanwhile,the underlying mechanism was revealed,originating from the destruction of the bacterial cell membranes and ROS generation.The SF-based coating was conducive to the adhesion,diffusion,and proliferation of bone marrow stem cells(BMSCs)on the surface,and promoted the expression of osteogenic genes and collagen secretion.The in vivo implantation results showed that compared with the untreated Ti implants,SF-based coating enhanced osseointegration at week 4 and 8.Overall,the AgNPs@AMPs-loaded SF-based coating presented the ability to synergistically inhibit bacteria and promote osseointegration,possessing tremendous potential application prospects in bone defects and related-infection treatments.

关 键 词：Antimicrobial peptides AGNPS ANTIBACTERIAL OSTEOINTEGRATION Titanium 

分 类 号：R318.08[医药卫生—生物医学工程]