Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiation  

作　　者：Xiaochan Li Boon Chin Heng Yunyang Bai Qianqian Wang Min Gao Ying He Xinwen Zhang Xuliang Deng Xuehui Zhang 

机构地区：[1]Department of Geriatric Dentistry,Peking University School and Hospital of Stomatology,Beijing,100081,PR China [2]Department of Dental Materials&Dental Medical Devices Testing Center,Peking University School and Hospital of Stomatology,Beijing,100081,PR China [3]Department of Prosthodontics,Peking University School and Hospital of Stomatology,Beijing,100081,PR China [4]Central Laboratory,Peking University School and Hospital of Stomatology,Beijing,100081,PR China [5]Center of Implant Dentistry,School and Hospital of Stomatology,China Medical University,Liaoning Provincial Key Laboratory of Oral Diseases,Shenyang,110002,PR China [6]National Engineering Research Center of Oral Biomaterials and Digital Medical Devices,NMPA Key Laboratory for Dental Materials,Beijing Laboratory of Biomedical Materials,Peking University School and Hospital of Stomatology,Beijing,100081,PR China

出　　处：《Bioactive Materials》2023年第2期81-92,共12页生物活性材料（英文）

基　　金：supported by the National Key Research and Development Program of China(2021YFB3800800,2021YFC2400400);the National Natural Science Foundation of China(Nos.82022016,81991505,51973004,52103312);the Beijing Municipal Natural Science Foundation(7222226);Peking University Medicine Fund(PKU2020LCXQ009).

摘　　要：Stem cells from human exfoliated deciduous teeth(SHED)uniquely exhibit high proliferative and neurogenic potential.Charged biomaterials have been demonstrated to promote neural differentiation of stem cells,but the dose-response effect of electrical stimuli from these materials on neural differentiation of SHED remains to be elucidated.Here,by utilizing different annealing temperatures prior to corona poling treatment,BaTiO_(3)/P(VDF-TrFE)ferroelectric nanocomposite membranes with varying charge polarization intensity(d_(33)≈0,4,12 and 19 pC N^(-1))were fabricated.Enhanced expression of neural markers,increased cell elongation and more prominent neurite outgrowths were observed with increasing surface charge of the nanocomposite membrane indicating a dose-response effect of surface electrical charge on SHED neural differentiation.Further investigations of the underlying molecular mechanisms revealed that intracellular calcium influx,focal adhesion formation,FAK-ERK mechanosensing pathway and neurogenic-related ErbB signaling pathway were implicated in the enhancement of SHED neural differentiation by surface electrical charge.Hence,this study confirms the dose-response effect of biomaterial surface charge on SHED neural differentiation and provides preliminary insights into the molecular mechanisms and signaling pathways involved.

关 键 词：Surface charge Electric polarization Dose-response effect Electrical microenvironment NEUROGENESIS 

分 类 号：TB33[一般工业技术—材料科学与工程]