针式滤器固相微萃取-酶抑制-数字图像比色法测定毒死蜱残留  

Determination of chlorpyrifos residues by needle filter solid phase microextraction-enzyme inhibition-digital image colorimetry

作　　者：郑晓娇 张欣悦 马祯 白冰 王晓闻[1] 荆旭 ZHENG Xiaojiao;ZHANG Xinyue;MA Zhen;BAI Bing;WANG Xiaowen;JING Xu(College of Food Science and Engineering,Shanxi Agricultural University,Jinzhong 030801,China;Institute of Forensic Science Public Security Bureau of Linfen,Linfen 041000,China)

机构地区：[1]山西农业大学食品科学与工程学院,晋中030801 [2]临汾市公安司法鉴定中心,临汾041000

出　　处：《农业工程学报》2023年第4期267-272,共6页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家自然科学基金(32202161);山西省重点研发计划项目(202102140601018)。

摘　　要：为解决食品中毒死蜱残留检测过程中步骤繁琐,灵敏度低的问题,采用高富集因子的针式滤器固相微萃取结合高灵敏度的酶抑制-数字图像比色法对毒死蜱进行快速检测。该研究以石墨烯纳米片作为固相吸附剂装入针式滤器,随后通过注射器重复抽打针式滤器的方法实现样品中毒死蜱的富集。酪氨酸酶能够将邻氨基苯酚和氯化铁体系氧化为黄色,毒死蜱会抑制酪氨酸酶的活性,从而会间接影响邻氨基苯酚和氯化铁体系的显色,通过数字图像比色法实现毒死蜱的定量分析。结果表明:毒死蜱在0.005~0.1 mg/L范围内线性良好,R^(2)>0.99。在矿泉水、茶水和果汁中的测定下限为0.005 mg/L。样品加标后的萃取率为86.6%~92.5%,相对标准偏差为3.2%~6.9%。该方法有助于开发农药的快速前处理和检测技术。Pesticides have been widely used to ensure the yield and quality of crops in modern agriculture,such as insecticides,herbicides,fungicides,and plant growth regulators.Among them,chlorpyrifos can be one of the best-selling organophosphorus pesticides in the world.However,the irrational use of chlorpyrifos can lead to excessive pesticide residues in food products,event harm to human health.The persistence and toxicity of chlorpyrifos have received ever-increasing attention in recent years.Furthermore,chlorpyrifos can be restricted or banned in a few countries,due to the residue risks.Therefore,the high-enrichment factor needle filter solid-phase microextraction and a highly sensitive enzyme inhibition-digital image colorimetry have been used for the rapid detection of chlorpyrifos residues in food samples,in order to avoid the traditional cumbersome steps and low sensitivity.However,there are still some challenges in both the pre-treatment and detection.In the pre-treatment process,the carbon materials as the solid phase adsorbents were added to a needle filter,and then the enrichment of chlorpyrifos in the food samples was achieved to repeat pumping of the needle filter by a syringe.The needle filter solid phase microextraction has the advantages of less adsorbent,less solvent,shorter time,and larger enrichment factors,compared with traditional solid phase extraction.The carbon material type and sample volume were optimized to achieve better recovery.In the detection,the tyrosinase can be used to oxidize the aminophenol and ferric chloride system into the yellow color.But chlorpyrifos can inhibit the activity of tyrosinase,even indirectly affect the color of the aminophenol and ferric chloride system.Particularly,digital image colorimetry can be used to analyze the color data after capturing the picture with a smartphone.The linear relationship can be quantitatively analyzed between chlorpyrifos concentration and green intensity.In this study,a highly efficient tyrosinase was selected,instead of acetylcholinestera

关 键 词：农药 萃取 酶抑制 针式滤器固相微萃取 数字图像比色法 毒死蜱 酪氨酸酶 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.3[轻工技术与工程—食品科学与工程]