高通量测序解析烟草苗床期基质细菌和真菌群落结构与多样性变化  

Community structure and diversity of bacteria and fungi in substrate of tobacco seedling based on high-throughput sequencing

作　　者：罗飞 李文红[2] 刘亭亭 汪汉成 孙美丽 蔡刘体 穆青 余知和[1] LUO Fei;LI Wenhong;LIU Tingting;WANG Hancheng;SUN Meili;CAI Liuti;MU Qing;YU Zhihe(College of College of Life Science,Yangtze University,Jingzhou,Hubei 434025,China;Guizhou Institute of Plant Protection,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;Guizhou Academy of Tobacco Science,Guiyang,Guizhou 550081,China;Qianxinan Municipal Company of Guizhou Tobacco Company,Xingyi,Guizhou,562400,China)

机构地区：[1]长江大学生命科学学院,湖北荆州434025 [2]贵州省农业科学院植物保护研究所,贵州贵阳550006 [3]贵州省烟草科学研究院,贵州贵阳550081 [4]贵州省烟草公司黔西南州公司,贵州兴义562400

出　　处：《中国烟草学报》2023年第2期79-88,共10页Acta Tabacaria Sinica

基　　金：国家自然科学基金项目(31960550);贵州省烟草公司黔西南州公司科技项目(州烟技(2021)4号2021-06);中国烟草总公司贵州省公司科技项目(201914,2020XM03)。

摘　　要：【背景和目的】烟苗的生长发育状况与育苗基质微生物密切相关,为了解烟苗育苗阶段基质细菌和真菌群落结构与多样性特征。【方法】采用IlluminaMiSeq高通量测序对育苗基质中细菌和真菌群落进行测序与分析。【结果】不同育苗阶段基质(播种后第10~66 d)真菌和细菌在菌群结构及相对丰度均存在差异。基质中优势细菌门均为变形菌门Proteobacteria(65.60%~72.80%)、拟杆菌门Bacteroidetes(16.75%~21.35%)和酸杆菌门Acidobacteria(1.38%~4.67%);优势细菌属均为德沃斯氏菌属Devosia(4.81%~14.20%)、罗河杆菌属Rhodanobacter(1.60%~6.19%)、戴氏菌属Dyella(0.38%~8.92%)等,其中,德沃斯氏菌属和罗河杆菌属在播种第66 d的相对丰度较第10 d分别降低8.6%和3.31%。播种第38 d基质细菌Shannon多样性指数和ACE丰富度指数最低,分别为7.25和1161.10;播种第24 d和66 d基质细菌Shannon多样性指数最高,分别为8.15和8.01。基质中优势真菌门均为子囊菌门Ascomycota(16.84%~23.55%)、担子菌门Basidiomycota(4.93%~11.95%)和Aphelidiomycota(0.04%~5.32%);优势真菌属均为假裸囊菌属Pseudogymnoascus(0.42%~11.63%)、节丛孢属Arthrobotrys(0.28%~14.29%)、革菌属Tomentella(0.21%~8.96%)等,其中,节丛孢属和革菌属在播种第52 d的相对丰度较第10 d分别提高14.01%和8.57%。播种第24 d基质真菌Shannon多样性指数最低,为3.93;播种第10 d和52 d基质真菌Shannon多样性指数最高,分别为5.57和5.86。功能预测显示,播种66 d内烟苗基质细菌主要功能分布在代谢类、遗传信息处理类和环境信息处理类等;真菌主要功能分布在腐生类和外生菌根类。【结论】烟草苗床期基质细菌和真菌群落种类无显著变化,但其相对丰度与多样性指数在不同育苗阶段存在差异。[Background and Objective] The growth,development and health status of tobacco seedlings are closely related to substrate microorganisms.This study aims to understand the community structure and diversity characteristics of bacteria and fungi in substrate of tobacco seedlings at seedling stages.[Methods] Illumina MiSeq sequencing was used to sequence and analyze the bacterial and fungal communities in substrate of tobacco seedlings.[Results] The structure and relative abundance of fungi and bacteria in substrate were different at the seedling stages(Days 10 to 66 after seeding).The dominant bacteriophyta in the substrate were Proteobacteria(65.60%-72.80%),Bacteroidetes(16.75%-21.35%) and Acidobacteria(1.38%-4.67%).The dominant bacteria genera were Devosia(4.81%-14.20%),Rhodanobacter(1.60%-6.19%) and Dyella(0.38%-8.92%),etc.Among them,the relative abundance of Devosia and Rhodobacter in the 66th day of sowing decreased by 8.6% and 3.31% as compared with that at the 10th day,respectively.At the 38th day of sowing,the Shannon diversity index and ACE richness index of bacteria in substrate were 7.25 and 1161.10.On the other hand,the highest Shannon diversity index was 8.15 and 8.01 at the 24th and 66th day of sowing,respectively.The dominant Eumycophyta were Ascomycota(16.84%-23.55%) and Basidiomycota(4.93%-11.95%) and Aphelidiomycota(0.04%-5.32%).The dominant fungi genera were Pseudogymnoascus(0.42%-11.63%),Arthrobotrys(0.28%-14.29%) and Tomentella(0.21%-8.96%),etc.Among them the relative abundance of Arthrobotrys and Tomentella at the 52th day of sowing increased by 14.01% and 8.57% as compared with that at the 10th day,respectively.The lowest diversity index of fungi in substrate was 3.93 at the 24th day of sowing.The highest Shannon diversity index was 5.57 and 5.86 at the 10th and 52nd days of sowing,respectively.The functional prediction showed that the main functions of bacteria in substrate were metabolic genetic information processing and environmental information processing within 66 days of sowing.Fungi we

关 键 词：苗床期 育苗基质 Illumina MiSeq高通量测序 群落结构 烟苗 微生物多样性 

分 类 号：S572[农业科学—烟草工业]