外源海藻糖对盐胁迫下番茄幼苗生长及抗氧化能力的影响  

Effects of exogenous trehalose on growth and antioxidant capacity of tomato seedlings under salt stress

作　　者：潘莹 巩文婷 廖伟彪 PAN Ying;GONG Wenting;LIAO Weibiao(College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学园艺学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2023年第1期69-76,共8页Journal of Gansu Agricultural University

基　　金：国家自然科学基金项目(31860568,31560563,31160398,32072559);国家重点研发项目(2018YFD1000800);甘肃省高等教育研究基金项目(2018C-14,2019B-082);甘肃省自然科学基金项目(1606RJZA073,1606RJZA077)。

摘　　要：【目的】探究外源海藻糖对盐胁迫下番茄幼苗生长和抗氧化机制的影响。【方法】以番茄(Solanum Lycopersicum L.‘Ailsa-Craig’)幼苗为材料,叶面喷施不同浓度的海藻糖溶液预处理2 d后进行盐胁迫,胁迫7 d后测定株高、茎粗等形态指标及相对电导率、丙二醛(MDA)和过氧化氢(H_(2)O_(2))含量等生理指标。【结果】不同浓度的海藻糖预处理有利于盐胁迫下番茄幼苗的生长,以10 mmol/L预处理效果最佳。与盐胁迫相比,10 mmol/L海藻糖处理下番茄株高和茎粗分别增长了28.8%和15.3%;鲜质量和干质量分别增加了15.4%和20.5%;叶面积和总根长增加了23.6%和23.1%。与盐胁迫相比,经过10 mmol/L预处理的番茄叶片相对电导率显著降低了11.4%;超氧阴离子(O_(2)^(-)·)、H_(2)O_(2)和MDA含量分别降低了17.8%、40.4%和26.6%。盐胁迫下,10 mmol/L海藻糖预处理过的番茄幼苗的抗氧化酶(SOD,POD,CAT和APX)活性均显著升高,抗氧化剂(ASA和GSH)含量分别显著升高了24%和7%。【结论】10 mmol/L的海藻糖预处理能显著提高番茄幼苗的抗氧化能力来缓解盐胁迫对番茄幼苗的氧化损伤,从而提高番茄幼苗的耐盐性。【Objective】The aim of this study was to investigate the effects of exogenous trehalose on the growth and antioxidant capacity of tomato seedlings under salt stress.【Method】Tomatoes(Solanum lycopersicum L.‘Ailsa-Craig’)were sprayed with different concentrations of trehalose.After 2 days of pretreatment,salt stress was applied.After 7 days of salt stress,plant height,stem diameter and other morphological indices as well as relative conductance,MDA and H_(2)O_(2) content and other physiological indices of tomato seedlings were determined.【Result】Different concentrations of trehalose pretreatment were beneficial for the growth of tomato seedlings under salt stress,10 mmol/L trehalose concentration being the best.Compared with the salt stress group,the plant height and stem diameter of tomato seedlings under 10 mmol/L trehalose concentration increased by 28.8%and 15.3%,respectively.Fresh weight and dry weight increased significantly by 15.4%and 20.5%,respectively.Leaf area and total root length increased by 23.6%and 23.1%,respectively.Compared to salt stress,the conductivity of tomato pretreated with 10 mmol/L trehalose decreased significantly by 11.4%.The contents of O_(2)^(-)•,H_(2)O_(2) and MDA were all lowest at the concentration of 10 mmol/L trehalose,which were 17.8%,40.4%and 26.6%lower,respectively,than in the salt stress group.The activities of SOD,POD,CAT and APX under 10 mmol/L trehalose treatment were significantly higher than those under salt stress.The contents of ASA and GSH in tomato leaves were significantly increased by 24%and 7%,respectively.【Conclusion】The concentration of trehalose at 10 mmol/L could significantly increase the activity of antioxidant capacity of tomato seedlings and alleviate the oxidative damage under salt stress,can improve the salt tolerance of tomato seedlings.

关 键 词：海藻糖 盐胁迫 番茄幼苗 生长发育 抗氧化能力 

分 类 号：S641.2[农业科学—蔬菜学]