‘海大2号’菠萝蜜茎尖组培褐变过程中酚类物质及相关酶活性的影响  

Effects of Phenolic Substances and Related Enzyme Activities in the Process of Tissue Culture Browning of Artocarpus heterophyllus Stem Tips of‘Haida 2’

作　　者：丁燕 郭佳慧 范紫云 向星星 干雨露 闵天雨 杨廷 丰锋[1] 叶春海[1] DING Yan;GUO Jiahui;FAN Ziyun;XIANG Xingxing;GAN Yulu;MIN Tianyu;YANG Ting;FENG Feng;YE Chunhai(College of Coastal Agriculture Sciences,Guangdong Ocean University,Zhanjiang,Guangdong 524088,China)

机构地区：[1]广东海洋大学滨海农业学院,广东湛江524088

出　　处：《热带作物学报》2023年第3期562-568,共7页Chinese Journal of Tropical Crops

基　　金：国家重点研发计划项目(No.2017YFD0202102);广东省科技计划项目(No.2016B020201010,No.2014A020208137)。

摘　　要：菠萝蜜因果实香甜深受大众喜爱,市场前景非常广阔,但常规种子繁殖难以保持其优良性状且嫁接法繁殖成活率极低。组培快繁技术在许多珍稀名优植物繁殖上已有广泛应用,但目前还没有应用于菠萝蜜生产实践的报道。组培过程中发现,菠萝蜜茎尖褐化严重,导致无性繁殖体系难以建立,为有效探究褐化的克服措施,促进菠萝蜜组培快繁技术体系的建立,为构建菠萝蜜种苗繁育技术体系及其产业化生产示范提供理论依据。以菠萝蜜品种‘海大2号’茎尖为外植体,利用不同时长低温预处理后将外植体接种至不同浓度的PVP培养基中对其进行初代培养,15 d后分别统计其褐化率和萌芽率,并通过测定其PPO活性变化、总酚及总黄酮含量,探究外植体经过低温预处理后不同浓度PVP对菠萝蜜茎尖组织培养中褐化的抑制情况。结果表明:茎尖经过12~24 h低温处理后再接入添加2.0 g/L PVP的1/2 MS培养基中,这一处理下菠萝蜜外植体的褐化率最低且萌芽率最高。且验证结果表明,此方法有利于菠萝蜜外植体的增殖。Artocarpus heterophyllus is popular for its sweet fruit and has a very promising market,but conventional seed propagation makes it difficult to maintain its excellent traits and the grafting method has a very low survival rate.The technique of tissue culture and rapid propagation has been widely used in the propagation of many rare and valuable plants,but there are no reports of its application in the production of A.heterophyllus.In the process of tissue culture,it was found that the stem tip of A.heterophyllus was severely browned,which led to the difficulty of establishing asexual reproduction system.In order to effectively investigate the measures to overcome the browning,this experiment took the stem tips of A.heterophyllus‘Haida 2’as the explants and inoculated them into different concentrations of PVP medium after low-temperature pretreatment at different lengths for primary culture,the browning rate and germination rate were calculated after 15 d,and PPO activity,total phenol and total flavonoid content were determined to explore the inhibi-tion of different concentrations of PVP on browning in stem the tip tissue culture of A.heterophyllus after low-temperature pretreatment.The results showed that the browning rate of A.heterophyllus explants was the lowest and the germination rate was the highest after 12‒24 h low-temperature treatment of stem tip and then in 1/2MS medium supplemented with 2.0 g/L PVP.The experimental results showed that this method was beneficial to the proliferation of A.heterophyllus explants.The purpose of this experiment is to promote the establishment of technique of tissue culture and rapid propagation for A.heterophyllus,with a view to providing a theoretical basis for the construction of the A.heterophyllus seedling breeding technology system and its industrial production demonstration.

关 键 词：菠萝蜜 组织培养 褐化 抗褐化剂 

分 类 号：S667.8[农业科学—果树学]