氮气诱导土沉香结香及挥发性成分分析  

Analysis of Agarwood Formation and Volatile Components of Aqui-laria sinensis Induced by Nitrogen Gas

作　　者：刘高峰 周再知[1] 黄桂华[1] 赵威威[1] 张青青 宋晓琛 LIU Gaofeng;ZHOU Zaizhi;HUANG Guihua;ZHAO Weiwei;ZHANG Qingqing;SONG Xiaochen(Research Institute of Tropical Forestry,Chinese Academy of Forestry,Guangzhou,Guangdong 510520,China;Heze University,Heze,Shandong 274015,China;Jiangxi Academy of Forestry,Nanchang,Jiangxi 330032,China)

机构地区：[1]中国林业科学研究院热带林业研究所,广东广州510520 [2]菏泽学院,山东菏泽274015 [3]江西省林业科学院,江西南昌330032

出　　处：《热带作物学报》2023年第3期638-646,共9页Chinese Journal of Tropical Crops

基　　金：广东省林业科技创新项目(No.2017KJCX001)。

摘　　要：以10年生土沉香树为材料,采用树干填充高压氮气方式,每隔7 d和15 d处理1次,连续处理3个月。分别在处理后7个月和10个月时取样,观察树干外部变色面积,测量树干木质部变色范围,检测醇溶性提取物和沉香四醇含量,并应用GC-MS分析所结沉香的化学成分及相对含量,探讨树干填充氮气对诱导土沉香的结香效应,为人工诱导土沉香结香提供可行的诱导方法。结果表明,氮气填充处理显著提高了土沉香木质部纵向变色长度和横向变色宽度,且每隔7 d填充1次气体(N_(7))的效果好于每隔15 d填充1次(N_(15))。处理结束后10个月时,N_(7)处理树干变色面积最大,且在纵向和横向上木质部沉香区和总变色长度均明显高于N_(15)和只打孔不充气处理(CK_(1))。氮气处理显著提高了土沉香醇溶性提取物和沉香四醇含量,且各处理沉香区含量均高于过渡区。处理结束后7个月时,N_(7)沉香区醇溶性提取物含量分别比N_(15)和CK_(1)高14.04%和28.47%,沉香四醇含量达0.12%,显著高于N_(15)和CK_(1)。随着处理时间的延长,各处理沉香区醇溶性提取物和沉香四醇含量均逐渐升高。处理结束后10个月时,N_(7)处理的沉香区醇溶性提取物和沉香四醇含量分别为19.37%和0.30%,明显高于N_(15)和CK_(1);N_(15)处理的醇溶性提取物和沉香四醇含量也高于CK_(1),分别比CK_(1)提高9.42%和51.50%。N_(7)处理的沉香区挥发物成分较多,富含25种萜烯类化合物、5种色酮类化合物以及8种其他类化合物,其中萜烯类和色酮类化合物总相对含量达66.09%,明显高于N_(15)和CK_(1)。The trunks of ten-year-old Aquilaria sinensis trees were filled with high-pressure nitrogen gas and treated every 7 and 15 days for 3 consecutive months.The wood discoloration area was observed and measured,the content of alcohol-soluble substances was detected,and the chemical composition and relative content of the agarwood were ana-lyzed by GC-MS at 7 and 10 months after treatment.The effect of nitrogen gas filled on the agarwood formation was discussed,which would provide a feasible induction method for artificial agarwood formation.The results showed that nitrogen treatment significantly increased the longitudinal discoloration length and transverse discoloration width of wood trunks,and the results of the treatment filled with nitrogen gas every 7 days(N_(7))were better than that of the treatment filled with nitrogen every 15 days(N_(15)).Ten months after N_(7)treatment,the discoloration area was the largest,and the length of agarwood zone and total discoloration zone in both longitudinal and transverse direction were signifi-cantly longer than those of N_(15)and CK_(1).Inflating nitrogen treatment could significantly increase the content of alcohol-soluble substances and agarotetrol,and the content of agarwood zone was higher than that of transition zone.The content of alcohol-soluble substances and agarotetrol in agarwood area increased by 14.04%and 28.47%compared with that of N_(15)and CK_(1),respectively,and the content of agarotetrol was 0.12%,which significantly higher than that of N_(15)and CK_(1),seven months after N_(7)treatment.The content of alcohol-soluble substances and agarotetrol in each treatment increased with the increase of treatment time.Ten months after N_(7)treatment,the content of alcohol-soluble substances and agarotetrol in agarwood zone was 19.37%and 0.30%respectively,which was noticeably higher than that of N_(15)and CK_(1).The content of alcohol-soluble substances and agarotetrol in N_(15)treatment increased by 9.42%and 51.50%re-spectively,compared with CK_(1).The volatile compon

关 键 词：土沉香 氮气 人工诱导 挥发性成分 

分 类 号：Q949.761.1[生物学—植物学] R284.1[医药卫生—中药学]