Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expression  

作　　者：Zhenhua Liu Mengke Wang Lijun Meng Yixiao Chen Qiuyi Wang Yan Zhang Xuefeng Xi Wenyi Kang 

机构地区：[1]National R&D Center for Edible Fungus Processing Technology,Henan University,Kaifeng 475004,China [2]Functional Food Engineering Technology Research Center,Kaifeng 475004,China [3]Joint International Research Laboratory of Food&Medicine Resource Function,Henan Province,Kaifeng 475004,China [4]Hebei Food Inspection and Research Institute,Hebei Food Safety Key Laboratory,Key Laboratory of Special Food Supervision Technology for State Market Regulation,Hebei Engineering Research Center for Special Food Safety and Health,Shijiazhuang 050017,China [5]College of Physical Education,Henan University,Kaifeng 475004,China

出　　处：《Food Science and Human Wellness》2023年第6期2014-2021,共8页食品科学与人类健康（英文）

基　　金：funded by National Key R&D Program of China(2022YFF1100300);National Natural Science Foundation of China(31900292);Science and Technology Development Program of Henan Province(212102110469,222102520035);Research on Precision Nutrition and Health Food,Department of Science and Technology of Henan Province(CXJD2021006).

摘　　要：Patrinia scabiosaefolia,is used as wild vegetable in China for more than 2000 years,with a variety of pharmacological activities,including anti-inflammatory,anti-tumor and hypoglycemic.Based on our ongoing research on chemical constituents and hypoglycemic activity of P.scabiosaefolia,4 lignan compounds,(+)-isolariciresinol(1),7R,7’R,8S,8’S-(+)-neo-olivil-4-O-β-D-glucopyranoside(2),4-O-methylcedrusin(3)and patrinian A(4),were isolated and identifi ed.The hypoglycemic activity showed that compounds 2 and 3 could extremely signifi cantly improve insulin resistance at 100(P<0.001),50(P<0.001)and 25μmol/L(P<0.01)in IR 3T3-L1 cells.While compound 4 only promoted glucose uptake by IR 3T3-L1 cells at 100μmol/L(P<0.01).Western blotting experiments showed that compounds 2 and 4 up-regulated the protein expressions of p-IRS,PI-3K,p-AKT and glucose transporter 4(GLUT4),and promoted the transcription of GLUT4 mRNA.Therefore,the mechanisms of compounds 2 and 4 were presumed to improve IR by activating PI-3K/AKT signaling pathway.

关 键 词：Patrinia scabiosaefolia Insulin resistance Patrinian A 7R 7’R 8S 8’S-(+)-neo-olivil-4-O-β-D-glucopyranoside 

分 类 号：R151[医药卫生—营养与食品卫生学]