Purifi cation and identification of anti-inflammatory peptides from sturgeon (Acipenser schrenckii) cartilage  

作　　者：Li Yuan Qian Chu Bei Yang Wei Zhang Quancai Sun Ruichang Gao 

机构地区：[1]School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China [2]Bio-resources Key Laboratory of Shaanxi Province,School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong 723001,China

出　　处：《Food Science and Human Wellness》2023年第6期2175-2183,共9页食品科学与人类健康（英文）

基　　金：supported by the China Agriculture Research System(CARS-46),China.

摘　　要：Cartilage is a nonedible byproduct with little saleable value.However,previous studies have proposed the possibility of producing peptides from cartilage with immune function modulation potential.The current study aimed to investigate the potential anti-inflammatory activity of peptides derived from sturgeon(Acipenser schrenckii)cartilage in lipopolysaccharide(LPS)-stimulated RAW264.7 macrophages.Five peptide sequences,including four novel peptides,were identified from ethanol-soluble cartilage hydrolysates.Among these five peptides,LTGP,LLLE,LLEL and VGPAGPAGP reduced the production of nitric oxide(NO)and interleukin-6(IL-6)while increasing interleukin-10(IL-10)excretion.Transcriptome analysis suggested the inhibition of activated mitogen-activated protein kinase(MAPK)and interleukin-17(IL-17)signaling pathways after LLEL intervention.MAPK,which is involved in the IL-17 signaling pathway,was further proved to be blocked by downregulating the phosphorylation of p38,extracellular-signal regulated protein kinase(ERK),and c-jun N-terminal kinase(JNK).This novel peptide offers an attractive approach to develop functional foods.

关 键 词：Sturgeon cartilage PEPTIDES ANTI-INFLAMMATORY Mitogen-activated protein kinase 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]