The specificity of ten non-digestible carbohydrates to enhance butyrate-producing bacteria and butyrate production in vitro fermentation  

作　　者：Jingjing Xu Ruyue Wang Weibao Liu Zhongwei Yin Jianrong Wu Xun Yu Wen Wang Hongtao Zhang Zhitao Li Minjie Gao Li Zhu Xiaobei Zhan 

机构地区：[1]Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China [2]Wuxi Second People's Hospital,Wuxi 214125,China [3]L&F Biotech.Ltd.7144 Collister Dr.Burnaby BC V5A3P6,Canada

出　　处：《Food Science and Human Wellness》2023年第6期2344-2354,共11页食品科学与人类健康（英文）

基　　金：supported by the National Key R&D Program of China(2021YFC2101100);Project funded by China Postdoctoral Science Foundation(2021M701463);the National Key R&D Program of China(2017YFD0400302);the National first-class discipline program of light industry technology and engineering(LITE2018-17);the Program of Introducing Talents of Discipline to Universities(111-2-06).

摘　　要：Butyrate and butyrate-producing bacteria are important indicators of gut microbial metabolism in human health.Ten non-digestible carbohydrates(NDCs),including inulin,fructooligosaccharide(FOS),oatsβ-glucans(OGS),oatsβ-glucan oligosaccharides(OGOS),Astragalus polysaccharides(APS),Astragalus oligosaccharides(AOS),xanthan gum oligosaccharides(XGOS),gellan gum oligosaccharides(GGOS),curdlan oligosaccharides(COS),and pullulan oligosaccharides(POS)were used to investigate NDC specifi city in modulating butyrate-producing bacteria and butyrate production in 48 h in vitro fermentation studies in combination with fecal inocula from 7 healthy donors and 11 patients with type 2 diabetes(T2D).We observed that the amount of these ten NDCs utilized depended on NDC structure and inter-individual gut microbial differences.XGOS and GGOS fermentations signifi cantly increased butyrate-producing bacteria(especially f_Lachnospiraceae)and butyric acid production.Furthermore,XGOS and GGOS fermentations showed a better ability to consistently modulate gut microbiota composition and metabolic properties between individuals of healthy donors or T2D patients when compared to inulin,FOS,APS,AOS,OGS,OGOS,COS and POS fermentation.This research indicated that xanthan gum and gellan gum oligosaccharides have strong specifi city to enhance butyrate-producing bacteria and butyrate production.

关 键 词：Non-digestible carbohydrates Gut microbiota Butyric acid Butyrate-producing bacteria 

分 类 号：TS201.3[轻工技术与工程—食品科学]